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PSC 533 DNA Facility
PSC 535 Cytometry Facility
PSC 537 Proteomics Facility
PSC 539 Dark Room
PSC 542
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PSC 545 Autoclaves
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PSC 561
PSC 563 Imaging
PSC 633
PSC 637
PSC 645 Autoclaves
PSC 657
PSC 659
PSC 659A Dark Room
PSC 659B Cell Sorting
PSC 661 Microarray Facility
PSC 835 Bioimaging Facility
PSC 839 Bioimaging Facility
PSC 873 Microscopy
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by Room: Petit Science Centre
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Image QuantTL Gel Analysis/Quantitation

Location: PSC 559, NSC 438, NSC 338,

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ImageQuant TL is the most automatic and easy-to-use general image analysis software available. It provides
• Automation for fast and consistent results.
• Optional ImageQuant SecurITy modules, designed to support and control traceability of data.
• Support of multicolor files.
• Editing tools to crop, rotate, and filter images.
• Advanced algorithms for delivery of accurate results.

Make: GE Healthcare

Form/Manual: Image QuantTL Gel Analysis/Quantitation Form

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Genespring Microarray Analysis

Location: PSC 559.

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Agilent's GeneSpring GX software provides powerful, accessible statistical tools for fast visualization and analysis of microarrays - expression arrays, miRNA, exon arrays and genomics copy number data. Designed specifically for the needs of biologists, GeneSpring GX offers an interactive desktop computing environment that promotes investigation and enables understanding of microarray data within a biological context.

Make: Agilent Technologies

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FlowJo Flow Analysis Software

Location: Dongle -Sign Out PSC 535 and PSC 559, NSC 338

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: FlowJo is a software package that is capable of analyzing flow cytometry data. All files produced by assorted flow cytometers are written in the Flow Cytometry Standard format with an .fcs file extension. FlowJo will import and analyze cytometry data regardless of which FACS (Fluorescence Activated Cell Sorting) insrument/software is used to collect the data.

To use the Software please contact Debby Walthall, who will explain how to download the software to your computer. The software access privileges are sometimes donglebased, and therefore will require you to sign-out for a dongle from PSC 521 or come to some other arrangements with Flow-Jo.

Make: FlowJo

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Decyder 2D Proteomics Analysis Software

Location: PSC 537

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: DeCyder 2-D Differential Analysis Software is specifically designed for 2-D fluorescence difference gel electrophoresis (DIGE) analysis and is a key element in the Ettan DIGE system (which we have). It is fullyully optimized and integrated to the 2-D DIGE platform.

Make: GE Healthcare Life Sciences

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ImageMaster 2D Analysis Software

Location: PSC 537, PSC 559

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ImageMaster 2D software offers a flexible interface for the comprehensive visualization, exploration, and analysis of 2-D gel data from both 2-D DIGE (2-D fluorescence difference gel electrophoresis) and non-DIGE technologies. It is provided as altrenative to the use of Decyder 2D amalysis Software

Make:GE Healthcare Life Sciences

Form/Manual: ImageMaster 2D Proteomics Analysis Manual

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Autoclave Steam

Location: PSC 545, PSC 645, PSC 745, NSC 484 and Kell Hall 445

Administrator: Gemeia Cameron or Sonja Young

Administrator Contact: 404-413-5363

Application: The importance of sterilization is essential to good laboratory practice, particularly in biotechnology, pharmaceutical and clinical laboratories. Of all sterilization methods, high pressure steam (autoclave) is the most widely used because of its efficacy, speed and reliability. The department has a number of autoclaves, some run by steam and others use steam, but the steam is generated by water loaded into the autoclave and electricity.

Autoclaves are used in a wide variety of applications including:

• culture media preparation
• labware sterilization
• waste sterilization

Make: Steris/AMSCO

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Autoclaves Electric

Location: PSC 645, NSC 336, NSC 484, STA Room 129

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The importance of sterilization is essential to good laboratory practice, particularly in biotechnology, pharmaceutical and clinical laboratories. Of all sterilization methods, high pressure steam (autoclave) is the most widely used because of its efficacy, speed and reliability. The department has a number of autoclaves, some run by steam and others use steam, but the steam is generated by water loaded into the autoclave and electricity. Autoclaves are used in a wide variety of applications including:

• culture media preparation
• labware sterilization
• waste sterilization

Make: Sanyo, Hirayama, and New Brunswick

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Aminco (Thermo) French Press

Location: PSC 545, NSC 368

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Thermo Electron offers the FRENCH Press and two models of FRENCH Pressure Cells for the disintegration of chloroplast materials, blood cells, unicellular organisms, homogenates of animal tissue and other biological particles. The pressure cell disrupts the cellular walls of a sample while leaving the cell nucleus undisturbed. This technique results in more uniform and complete disruption. The FRENCH Pressure Cell delivers higher pressures than bombs or decompression systems, without pre-treating the sample. Cells are lysed by forcing the cell or tissue suspension through a narrow space, thereby shearing the cell membraneøs.A French press consists of a piston that is used to apply high pressure to a sample volume of 40 to 250 ml, forcing it through a tiny hole in the press. It can reach pressures of 20,000-40,000 pounds per square inch (psi).

Make: Thermo Electron

Form/Manual: French Press Operation Manual

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Sonifier/Cell Disrupter (Tip)

Location: PSC 545, NSC 484 (Heat Systems -Ultrasonic)

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: The Sonifier® ultrasonic cell disruptors are versatile laboratory tools designed to apply high-frequency ultrasonic energy to biological and chemical processing to provide:

• Cell disruption
• Nano particles
• Emulsifications
• Homogenization
• Cell lysis
• Processing DNA & proteins
• Fine mixing
• Degassing

Make: Fisher / Emerson Industrial Automation

Form/Manual: Sonicator Tip Operations Manual

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Sonifier/Cell Disrupter (Bath)

Location: PSC 645, NSC 484

Administrator: Hyuk_Kyu Seoh

Administrator Contact: (404) 413-5379

Application: The Sonifier® ultrasonic cell disruptors are versatile laboratory tools designed to apply high-frequency ultrasonic energy to biological and chemical processing to provide:

• Cell disruption
• The bath adapter allows for the simultaneous sonication. didruption of cells surrounded by ice water, to • unify cell disruption

Make: Fisher / Emerson Industrial Automation

Form/Manual: Sonicator (Bath) Operations Manual

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KDL Agitator Bead Mill

Location: NSC 480 (Fermentation Room)

Administrator: George Pierce

Administrator Contact: 404-413-5315

Application: This system allows for continuous temperature control constantly maintaining cell suspensions at a low temperature. It offers a controlled cell breakage rate, and direct steam sterilization and flushing with antiseptics (DynoMill brochure).

Make: DynoMill

Form/Manual: KDL Agitator Bead Mill Service Form

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Avanti J-20 Floor

Location: PSC 637

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-20 has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-20 Floor Operation Manual

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Avanti J-26 XP Floor Centrifuge

Location: PSC 659

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-26 XP has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-26 XP Floor Operation Manual

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Avanti J25 Floor Centrifuge

Location: NSC 340

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-25 has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-25 Floor Operation Manual

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Avanti J25-I Floor Centrifuge

Location: PSC 543

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-25I has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-25-I XP Floor Operation Manual

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Sorvall RC5B Floor Centrifuge

Location: NSC 460, Kell 445 (Sorvall RC2B)

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The Sorvall RC5B can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Sorvall

Form/Manual: Operation Manual

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Eppendorf 5804 R Tabletop Centrifuge

Location:PSC 543, PSC 637 and NSC 448

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Centrifuge 5804 R is a high speed, refigerated centrifuge for medium capacity needs. It allows for molecular applications in tubes up to 250 mL and offers additional swing-bucket and fixed-angle rotors as well as deepwell plate capacity for increased versatility.

Make: Eppendorf

Form/Manual: 5804-Refrigerated Tabletop Operation Manual

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5810 R Tabletop

Location: PSC 555

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Centrifuge 5810 and 5810R are -high speed centrifuges for medium capacity needs. They allow for molecular applications in tubes up to 250 mL and offer additional swing-bucket and fixed-angle rotors as well as deepwell plate capacity for increased versatility.

Make: Eppendorf

Form/Manual: 5810 R Tabletop Operation Manual

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5430 R Tabletop

Location: PSC 533, NSC 338 and NSC 488

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The Centrifuge 5430 R combines the proven features of the Eppendorf microcentrifuges and the multipurposecentrifuges. They offer multiple application opportunities due to a high selection of different rotor types. Despite their compact size of approx. 35 cm width, special rotors for micro plates, 15 mL and 50 mL conical tubes can be easily used. Their incomparable versatility makes the Centrifuge 5430 and Centrifuge 5430 R the flagships under the Eppendorf microcentrifuges.

Make: Eppendorf

Form/Manual: 5430 R Tabletop Operation Manual

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AKTA Explorer

Location:PSC 542A Cold Room, PSC 657

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ÄKTA™Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form/Manual:

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AKTA Purifier

Location: PSC 559, PSC 5th floor Cold Room, Rm 657, STA Turners Suite #1

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:ÄKTA™Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form/Manual:

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MDQ

Location: PSC 637A -needs to be prepped

Administrator: Hyuk Kyu Seoh

Administrator Contact: (404) 413-5379

Application: The MDQ is a capillary-based analytical system separates sample components within a fused-silica capillary, using one of several modes of electrophoresis.

Applications: Examples of the different modes of electrophoresis include:
• Capillary Zone Electrophoresis
• Micellar Electrokinetic Capillary Chromatography
• Capillary Gel Electrophoresis
• Capillary Isoelectric Focusing
• Capillary Electrochromatography.
The system includes a P/ACE MDQ configured with both a photo diode array and selectable-wavelength UV/Vis (200, 214, 254 and 280 nm filters included) detector, UV source optics, temperature-controlled sample storage module and 32 Karat™ Software. Typical applications are:
• Genetic analysis
• Chiral analysis
• Fast RNA purity analysis
• Basic drugs analysis
• Ion analysis
• Protein and Peptide analysis

Make: Beckman

Form/Manual: MDQ Service Form

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HPLC

Location: PSC 9th Floor

Administrator: Hyuk Kyu Seoh

Administrator Contact: 404-413-5379

Application: The Beckman HPLC has solvent delivery modules that are built with a rapid fill pump design that produce smooth flow from microbore to semi-preparative flow rates and thee high-pressure mixing design eliminates the need for constant helium sparging or degassing. The pumps are combined with high-sensitivity UV/VIS and diode array detectors and autosamplers. Flow range: 0.01 - 10.0 ml/min.; wavelength range: 190 - 700 nm; Maximum back pressure: 17 ul capacity flow cell; Programmable functions: wavelength, range, rise time, auto zero, event mark, relays, lamp off, peak detection, peak threshold, bandwidth and stop data.

Preparative HPLC refers to the process of isolation and purification of compounds. Important is the degree of solute purity and the throughput, which is the amount of compound produced per unit time. This differs from analytical HPLC, where the focus is to obtain information about the sample compound. Chemical Separations can be accomplished using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase. Each compound should have a characteristic peak under certain chromatographic conditions. Depending on what needs to be separated and how closely related the samples are, the chromatographer may choose the conditions, such as the proper mobile phase, to allow adequate separation in order to collect or extract the desired compound as it elutes from the stationary phase. With these characteristic peaks, identification of compounds can be done. HPLC also allows for quantification of compounds by determining the unknown concentration of a compound in a known solution.

Make: Beckman

Form/Manual: HPLC Service Form

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SpeedVac -Concentrator

Location: PSC 555, NSC 338, NSC 460, Kell 405

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The Concentrator plus from Eppendorf is available in two configurations:

Complete system with an integrated service-free diaphragm pump
Basic concentrator for connecting to an existing vacuum.
The Concentrator’s heating technology optimizes the evaporation process. No matter which configuration you choose, you’re assured of quick and efficient—yet gentle—vacuum concentration of your samples (e.g. DNA/RNA, proteins).

The complete system includes a vapour condenser that purifies the exhaust air by up to 85%. This serves as protection against unpleasant vapours.

Make: Eppendorf

Form/Manual: Speed Vac Concentrator Operation Manual

labcommander
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Vacufuge

Location:PSC 543, NSC 338, NSC 460, Kell 405

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The Vacufuge Concentrator is a complete system with solvent-resistant PTFE vacuum pump and vapor trap. Narrow, 13-inch width saves valuable bench space. Motorless drive and sealed chamber provide trouble-free operation Evaporation Modes:

• Choice of three (aqueous, alcohol, off) to correspond with sample solvent and reduce processing time up to 20%.
• Maximize evaporation efficiency and prolong pump lifespan by periodically aerating the vacuum line, preventing condensation build-up.

Automatic vacuum release prevents sample loss from bumping and foaming. Brake feature brings rotor to a faster stop—allows quick access to samples. Choice of four heating levels (30°C, 45°C, 60°C, and ambient) allows safe and efficient concentration of a variety of samples. Besides the microfuge tube rotor, there is a micro plate rotor available upon request (see Debby).

Make: Eppendorf

Form/Manual: Vacufuge Operation Manual

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Steris Dishwashers

Location: PSC 545, PSC 645, PSC 745, NSC 336, NSC 484 and Kell 445

Administrator: Gemeia Cameron

Administrator Contact: (404) 413-5379

Application: Washing Dishes

Make: Steris/AMSCO

Form/Manual: Dishwasher Operation Manuals

labcommander
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DNA Sequencer 3730

Location: PSC 533

Administrator: Ping Jiang

Administrator Contact: (404) 413-5370

Application:The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Make: Life Technologies

For Use and Further Information: DNA Sequencing Services

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Ion One-Touch Chip Prep'n Station

Location: PSC 533

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Ion OneTouch™ 2 System simplifies the workflows for the Ion Proton™ and Ion PGM™ systems by providing an automated solution for scalable and reproducible template preparation. The Ion OneTouch™ 2 System: • Integrates multiple manual template preparation workflows into a single system
• Enables parallel processing of multiple samples per day through modular design
• Supports scalable template preparation for all Ion semiconductor sequencing chips
• Supports up to 400 bases on the Ion PGM™ System and up to 200 bases on the Ion Proton™ System*
• Includes a full thermal cycling modality for amplification for quick optimization of thermal cycling parameters
• Employs a peristaltic pump fluidic system that eliminates the need for gas
• Fits easily on any benchtop with its small instrument footprint

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

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Ion PGM System Next-Gen. Sequencer

Location: PSC 533

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Personal Genome Machine (PGM™) System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any lab or clinic. The system leverages the exponential improvements in the semiconductor industry (known as Moore’s Law) to provide scalability and flexibility for various applications. The system’s use of the simplest, natural sequencing chemistry eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension. Direct, real time sequencing detection provides sequencing results typically in less than 3 hours.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

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Ion-One Touch Prep Station

Location: PSC 533

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Ion Chef™ System is the next generation of workflow simplification products for the Ion Proton™ and Ion PGM™ systems. The Ion Chef™ System provides automated, high-throughput template preparation and reproducible chip loading for users at any experience level. With typically less than 15 minutes of hands-on time, an intuitive touch-screen interface, and significantly higher productivity than the Ion OneTouch™ 2 System, the Ion Chef™ System makes the Ion Torrent™ sequencing workflow accessible for every lab.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

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Ion Proton System; Next-Gen. Sequencer

Location: PSC 533

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Ion Proton™ System offers a complete workflow from sample through analysis, and variant calling or RNA analysis. It is used with the Ion Chef™ System* to enable automated, high-throughput template preparation and reproducible chip loading for users of any experience level. Built for speed, the Ion Proton™ System provides sequencing run times from 2–4 hours on the Ion PI™ Chip, taking you from DNA to variants in a single day. It is equipped with Ion Reporter™ Software, providing integrated tools for tertiary data analysis for DNA variation analysis across single, paired, or trio research samples.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

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CHEF-DR II Pulse Wave

Location: NSC 340

Administrator: Debby Walthall

Administrator Contact: 404-413-5363

Application: This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system is optimized for the most popular range of separations with a fixed angle of 120°. This unit can be used for separations of fragments up to 6 Mb in size by adjusting the running conditions for low voltage and extended run times. Besides mapping, this system can be used for:

• Strain typing-molecular epidemiology
• Apoptosis assays
• DNA damage and repair studies
• Large protein separations

Pulsed Field Gel Electrophoresis Is Still the Benchmark for Mapping Applications such as: • Disease locus mapping

• YAC, BAC, PAC, and cosmid mapping
• Chromosome rearrangements
• RFLP and DNA fingerprinting

Make: BioRad

Form/Manual: CHEF DR II Pulse Wave

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Protein Simple Simon Western

Location: PSC 537

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: Simple Western by ProteinSimple effectivley reinvents the entire Western blot process without gels, transfer tanks, blots or film - finally giving researchers a complete, walk-away solution for protein detection and characterization. All hands-on steps from sample loading and detection to quantitative data analysis are completely automated, eliminating the manual factors that can negatively impact reproducibility, quantitation, time to result and data reliability. Simon analyzes 12 samples per run in 3-5 hours! Funding provided by Tech fee.

Make: Protein Simple

Form/Manual: Protein Simple Simon Western

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Gene Pulser Xcell

Location: STA Turner Suite Rm #3

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application:T he Gene Pulser Xcell™ system is a modular electroporation system for transfecting every cell type. The system includes a main unit, a ShockPod™ cuvette chamber, and your choice of accessory modules: the capacitance extender (CE module) and the pulse controller (PC module).

Features and Benefits:
• Universal electroporation — transfects all cell types, from primary and stem cells to bacteria and yeast
• Preset protocols — include the most common mammalian and bacterial cell types
• Flexibility — choice of programs for preset protocols, optimization protocols, manual operation, and/or user protocols
• Protocol library — collection of electroporation protocols for every cell type including primary, • immortal, and bacterial cells
• Data management — enables storage and recall of parameters used in the previous 100 experiments, useful for troubleshooting
• Reproducibility — uses PulseTrac™ circuitry and arc-protection features to ensure reproducibility and sample protection

Make: BioRad

Form/Manual: Gene Pulser Xcell

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Micropulser

Location: PSC 543, PSC 545

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: The MicroPulser electroporator is a simple yet versatile instrument that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms. Transformation efficiencies much higher than those obtained with chemical methods can be achieved.
Key Features:
• One-button pulse delivery, attached cuvette chamber, and rapid charge time for fast sample handling
• Preset, optimized programs for commonly studied bacteria and fungi allow rapid program selection
• Arc quenching (ARQ) system significantly reduces arcing, protecting against loss of valuable samples
• Broad range of parameters for manual optimization. Manual programming allows voltage to be selected • in a 200–3,000 V range with 10 V precision and allows pulse width to be selected in a 1.0–4.0 ms range with 0.1 ms precision
• High-voltage 3,000 V capability yields improved efficiency in larger-volume cuvettes
• Compact, space-saving design
• Audible and visible pulse indicators
• Time constant and actual volts displayed to monitor reproducibility

Make: BioRad

Form/Manual: Micropulser Operation Manual

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Kodak X-Omat 2000A

Location:PSC 539, PSC 659A, NSC 473 and

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The X-OMAT 2000A processes exposed radiographic film. The exposed film sheet is placed into an automatic film processor. The film is processed via rollers through tanks containing developer and fixer solutions, and finally through a wash system. The developed film emerges from the processor ready for reading by the researcher.

Make: Kodak

Form/Manual: X-Omat 2000A Operation Manual

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Accuri C-6 Blue/Red

Location: NSC 338A

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: The BD Accuri™ C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable. The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. As a result, flow cytometry has become a staple of modern laboratories around the world.

Make: BD Biosciences

Form/Manual: Accuri C-6 Blue/Red

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Aria Cell Sorter

Location: PSC 659B

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: This benchtop system delivers high-speed sorting and multicolor analysis. The BD FACSAria instrument is the first benchtop sorter that incorporates a fixed-alignment cuvette flow cell, which minimizes startup time and improves reproducibility. This new flow cell provides superior fluorescence sensitivity and freedom from instrument maintenance and alignment. Gel coupling to the objective lens improves collection efficiency and increases sensitivity and resolution needed ofr multicolor sorting applications. Additional "Sort setup" and "sort monitoring" features in the system software makes the BD FACSAria instrument easy to use. BD FACS™ Accudrop technology is integrated into the system for quick and accurate drop-delay determination. Stream monitoring of the breakoff point and clog detection are incorporated. The system has four air-cooled lasers at 488-nm, 633-nm, 405-nm and 355-nm wavelengths. Two- and four-way bulk sorting devices for a variety of tube sizes are also available. Water Jacketed tube holders are also available to keep cells warm or cold as needed.

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

For Use and Further Information: Flow Cytometry and Cell Sorting Services

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FACSCanto II

labgate
Location: PSC 535

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results.

The BD FACSCanto II provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors.*

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

For Use and Further Information: Flow Cytometry and Cell Sorting Services

labcommander
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Cellometer Cell Counter

Cellometer Auto2000

Location: PSC 637, NSC 338

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: The Auto 2000 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Make: Nexcelom

Form/Manual: Cellometer Cell Counter

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LSR Fortessa

labgate
Location: PSC 535

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

The Fortessa is configurable and upgradeable with up to 4 lasers to detect up to 18 colors simultaneously, BD LSR analyzers feature innovative designs for both the excitation optics and collection optics that reduce excitation losses and improve light collection efficiency. Our instrument has blue (488 nm), red (633 nm), yellow-green (561 nm) and violet (405nm) lasers. The addition of the yellow-green laser allows use of the new fruit fluorescent proteins such as mCherry. It also limits spectral overlap between FITC and PE – 2 of the most common fluorescent markers used in flow cytometry.

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

For Use and Further Information: Flow Cytometry and Cell Sorting Services

labcommander
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-80ºC Freezers

Location:PSC 54, PSC 559, PSC 637, PSC 659, NSC 488, NSC 340, Kell 405

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application: Maintain viable stocks and strains at -80°C

Make: Thermo

Form/Manual: Freezer Manual

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Explosion-Proof -20°C Freezer

Location: PSC 543

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application: Explosion Proof -20ºC

Make: Thermo

Form/Manual: Freezer

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BT3.3 EL Lyophilizer Tabletop

Location: PSC 555

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: 404-413-5363 and 404-413-5379

Application: Sublimation occurs when a frozen sample goes directly to the gaseous phase without passing through the liquid phase. As the ice sublimates, it leaves voids in the dry residual material making it easy to rehydrate. Freeze drying samples stabilizes them for storage, preservation, or shipping . Many times, drying causes damage and some loss of cellular or protein activity. Lyophilization is a method of sample preservation that significantly reduces damage to biological samples. Substances that are not damaged by freezing can usually be lyophilized so that refrigerated storage is unnecessary. Important exceptions are mammalian cells, nearly all of which are destroyed by lyophilization. However, many microorganisms and proteins survive lyophilization well, and it is a preferred method of drying vaccines, pharmaceuticals, blood fractions, and other laboratory samples. Some specialist food products are also lyophilized.

Make: Virtis

Form/Manual: BT3.3 EL Lyophilizer Tabletop Service Form

labcommander
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Lyophilizer Freezone Large

Location: NSC 460

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: Sublimation occurs when a frozen sample goes directly to the gaseous phase without passing through the liquid phase. As the ice sublimates, it leaves voids in the dry residual material making it easy to rehydrate. Freeze drying samples stabilizes them for storage, preservation, or shipping . Many times, drying causes damage and some loss of cellular or protein activity. Lyophilization is a method of sample preservation that significantly reduces damage to biological samples. Substances that are not damaged by freezing can usually be lyophilized so that refrigerated storage is unnecessary. Important exceptions are mammalian cells, nearly all of which are destroyed by lyophilization. However, many microorganisms and proteins survive lyophilization well, and it is a preferred method of drying vaccines, pharmaceuticals, blood fractions, and other laboratory samples. Some specialist food products are also lyophilized.

Make: LabConco

Form/Manual: Freezone Large Service Form

labcommander
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Lyophilizer Freezone 12

Location: NSC 460

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: Sublimation occurs when a frozen sample goes directly to the gaseous phase without passing through the liquid phase. As the ice sublimates, it leaves voids in the dry residual material making it easy to rehydrate. Freeze drying samples stabilizes them for storage, preservation, or shipping . Many times, drying causes damage and some loss of cellular or protein activity. Lyophilization is a method of sample preservation that significantly reduces damage to biological samples. Substances that are not damaged by freezing can usually be lyophilized so that refrigerated storage is unnecessary. Important exceptions are mammalian cells, nearly all of which are destroyed by lyophilization. However, many microorganisms and proteins survive lyophilization well, and it is a preferred method of drying vaccines, pharmaceuticals, blood fractions, and other laboratory samples. Some specialist food products are also lyophilized.

Make: LabConco

Form/Manual: Freezone Large Service Form

labcommander
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Freeze Dry System 4 Small

Location: Kell Hall 405

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: LabConco

Form/Manual: Freeze Dry System 4 Small Service Form

labcommander
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Lyophilizer Freezone Plus 4.5

Location: PSC 555

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: LabConco

Form/Manual: Lyophilizer Small Service Form

labcommander
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Lyophilizer Small w/ Dry Ice Trap

Location: PSC 637

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: LabConco

Form/Manual: Lyophilizer Small with Dry Ice Tap Service Form

labcommander
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BioRad Gel Dryer w/ Dry Ice Trap

Location: Kell Hall 405

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Model 583 Gel Dryer allows the drying of acrylamide and other gel types.
The Model 583 gel dryer has a 35 x 45 cm drying surface, which is large enough to fit up to 16 mini gels, 9 Criterion gels, 2 large format gels, or 1 sequencing gel.

Make: BioRad

Form/Manual:

labcommander
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Affymetrix Microarray

Location: PSC 661

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: Affymetrix

For Use and Further Information: Genomics Microarray Services

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Fluidics Station 450

Location: PSC 661

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The GeneChip® Fluidics Station 450 is used for the wash and stain operation of Affymetrix GeneChip® arrays. The instrument includes design advancements to provide improved ease of use and true walk-away freedom dramatically improving efficiency in your genetic analysis.

Make: Affymetrix

For Use and Further Information: Genomics Microarray Services

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Hybridization Oven

Location: PSC 661

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: GeneChip® Hybridization Oven provides uniform hybridization environmment for consistent RNA : chip bound DNA interactions, allowing for appropriate discrimination between specific and background signals, and between closely related target sequences.

Make: Affymetrix

For Use and Further Information: Genomics Microarray Services

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Biolog Phenotype Microarray

Location: PSC 542 Cold Room -storage

Administrator: Chung Dar Lu and P.C. Tai

Administrator Contact: (404) 413-5363

Application: DNA Microarrays and Proteomic Technologies allow scientists to detect genes or proteins that are coregulated and whose patterns of change correlate with something important such as a disease state. However there is no assurance that these changes are really significant to the cell. Phenotype MicroArrays are a complementary technology providing the needed information at the cellular level ... and much more. After a genetic change or exposure to a drug lead, the researcher can directly evaluate the cellular response to that change. Phenotype MicroArrays can monitor, either directly or indirectly, most known aspects of cell function. The range of phenotypes includes:

• Cell surface structure and transport functions
• Catabolism of carbon, nitrogen, phosphorus, and sulfur
• Biosynthesis of small molecules
• Synthesis and function of macromolecules and cellular machinery
• Cellular respiratory functions
• Stress and repair functions
• Other cellular properties

Make: Biolog

Form/Manual: Biolog Phenotype Microarray

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GenePix Microarray Scanner

Location:PSC 559

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The popular GenePix® 4000B Microarray Scanner has earned its outstanding reputation due to its incredible, intuitive, and reliable operation. Our commitment to engineering excellence guarantees that the GenePix 4000B Microarray Scanner remains the benchmark in microarray scanning technology. In 2010 we updated the electronics to provide a state-of-the-art microarray scanner with the finest quality components while maintaining the features and performance that you have become accustomed to.

Make: Molecular Devices

Form/Manual: GenePix MicroArray Scanner

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Histocenter 3

Location: NSC 448

Administrator: Robert Simmons and Sonja Young

Administrator Contact: (404) 413-5363

Application: The Histocenter 3 embeds tissue samples into paraffin blocks for microtome sectioning.

Make: Shandon

Form/Manual: Histocentre 3

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HM 550 Microtome

Location: NSC 448

Administrator: Robert Simmons and Sonja Young

Application: The HM550 Microtome esnures that sectioning of paraffin blocks into extremely thin slices proceeds at an optimal sectioning temperature

Make: Fisher

Form/Manual: HM 550 Microtome

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Microtome, Model 860 Sliding

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: IMEB.inc

Form/Manual: Microtome Model 86C Sliding

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Rotory Microtome

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: Unknown

Form/Manual: Rotory Microtome Service Form

labcommander
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STP-120 Spin Processor

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: The STP-120 Spin Processor embeds the tissue in paraffin.

Make: Fisher

Form/Manual: STP-120 Spin Processor Service Form

labcommander
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Bioanalyzer 2100

Location: PSC 533

Administrator: Ping Jiang

Administrator Contact: 404-413-5370

Application: The Bioanalyzer 2100 provides for a rapid visualisation of sample quality and quantity. High sensitivity with minimal sample quantities. Significant time savings - up to12 samples in 30 minutes. Reduced use and waste of hazardous chemicals.

Make: Agilent

Form/Manual: Bioanalyzer 2100

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Qubit 3 Fluorometer

Location: PSC 533

Administrator: Ping Jiang

Administrator Contact: 404-413-5379

Application: The Qubit™ 3.0 Fluorometer is the next generation of the popular benchtop fluorometer that accurately measures DNA, RNA, and protein using the highly sensitive Qubit quantitation assays. The concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to to the target, which minimizes the effects of contaminants—including degraded DNA or RNA—on the result. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds Key features of the Qubit 3.0 Fluorometer include: Powerful, dual-core processor quickly and accurately quantifies DNA, RNA, and protein, in less than 5 seconds per sample Uses as little as 1 μL of sample Stores up to 1,000 sample results Large 5.7-inch state-of-the-art color touch screen for easy workflow navigation Ability to personalize your Qubit Fluorometer with the assays you run most, add new assays, or even create your own with MyQubit

Make:Thermofisher

Form/Manual: Qubit 3 Fluorometer

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LAS4000 mini Chemiluminescence

labgate
Location: PSC 559 NSC 438

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Exceptional sensitivity for quantitation of biomolecules labeled with chemiluminescent probes Supports gel documentation of Coomassie™ blue and silver-stained samples. Accurate quantitation of closely spaced bands in complex protein samples for gels and films up to 18 x 12 cm. Low noise for longer exposure times and lower background, important for precise quantitation of very weak signals. Wide linear dynamic range for precise quantitation of faint and dark bands over four orders of magnitude in the same image, avoiding time-consuming multiple film exposures. Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision and uniform quantitation. Sensitive detection of less than 40 pg of protein on a Western blot. Sensitivity may be increased by "binning" of up to 8 x 8 pixels and smoothing. Chemiluminescent and colorimetric signals can be captured in the same image without changing the lens.

Make: GE Healthcare

Form/Manual: LAS4000 mini Chemiluminescence

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Amersham Imager 600

Location: STA Room 138

Administrator: Debby Walthall

Administrator Contact: 404-413-5379

Application: Amersham Imager 600 is a sensitive and robust chemiluminescence imager for high-resolution digital imaging of protein and DNA samples in gels and membranes. Intuitive operation: You can operate the instrument from a tablet computer with easy-to-use image analysis software. No prior imager experience or training is required to obtain high-quality results. Excellent performance: Advanced optics provides high-resolution images, high sensitivity, and broad dynamic range. Robustness: An optimal instrument for multi-user labs.

Make: GE Healthcare

Form: LAS4000 mini Chemiluminescence Service Form

labcommander
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Omega 10g EtBr OK

Location: NSC 338 (EtBr OK), Kell 405 (No EtBr)

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Some applications on the Omega 10g are SYBR Green stained gels and GFP expressed experiments and Qdot multi-plexed western blots. The instrument has megapixel resolution, touch pad control panel, adjustable height sample tray, variable intensity UV Transilluminator and multi-wavelength LED Epi-Illumination.

Make: Ultra Lum

Form/Manual: Omega 10g

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Typhoon Trio Phospho, Fluorescent

labgate
Location: PSC 537

Administrator: Hyuk-Kyu Seoh and Debby Walthall

Administrator Contact: 404-413-5379 and 404-413-5363

Application: The Typhoon Trio can image Store phosphor autoradiography and many types of fluorescent stains (direct blue-excited fluorescence – 457 and 488 nm; direct green-excited fluorescence – 532 nm; direct red-excited fluorescence – 633 nm. This imager is specifically designed for 2-D DIGE multicolor scanning and detection of CyDye DIGE Fluor dye-labeled proteins. Other common fluorescent stains that this imagers can image are Deep Purple, Coomasie Blue, EtBr, SYPRO red and SYPRO Ruby. They can image agarose and acrylamide gels, blots, microarray slides and imaging plates of many different sizes.

Make: GE Healthcare

Form/Manual: Typhoon Trio Phospho, Fluorescent

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UVP BioDoc-it® No EtBr

Location: PSC 543, 659, NSC 338,

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, Autorad film, colony plates and Fluorescent Gels. This self-contained gel imaging system features a scientific grade 2.0 megapixel CCD camera for capturing high resolution images with simple yet versatile capture and save options. Screen tilts to optimum viewing angle. A built-in computer touch screen supplies a complete system at one location for fluorescent and colorimetric imaging, while the attached comp.

NO ETHIDIUM BROMIDE is to be used in this instrument

Make: UVP

Form/Manual: UVP old EtBr OK Service Form

labcommander
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UVP BioDoc-it® EtBr use OK

UVP
Location: PSC 637

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, Autorad film, colony plates and Fluorescent Gels. This self-contained gel imaging system features a scientific grade 2.0 megapixel CCD camera for capturing high resolution images with simple yet versatile capture and save options. Screen tilts to optimum viewing angle. A built-in computer touch screen supplies a complete system at one location for fluorescent and colorimetric imaging.

Make: UVP

Form/Manual: UVP EtBr OK

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SynGene InGenius® EtBr use OK

Syngene
Location: STA Turners Suite #2

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, colony plates and Fluorescent Gels. InGenius3 is a self-contained, low budget gel documentation and analysis fluorescence applications. It features a compact darkroom which has a sliding front door and an internal LED white light. InGenius3 has a 3m pixel camera and can produce images with 16 bit image depth. A manual zoom lens and manual filter drawer (includes a UV filter) completes the package.

Make: Syngene

Form: UVP EtBr OK Service Form

labcommander
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FLA 7000 Phospho/ Fluorescent Imager

Location: STA: Turner Suite, Rm #1

Administrator: Hyuk-Kyu Seoh and Debby Walthall

Administrator Contact: 404-413-5379 and 404-413-5363

Application: The FLA 7000 is an advanced, multipurpose bio-imaging analyzer which enables radioisotopic, fluorescent and digitized image capturing.

Make: GE Healthcare

Form/Manual: FLA7000FLA 7000 Phospho / Fluorescent Imager

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Typhoon 9400 Imager

labgate
Location: NSC 438

Administrator: Hyuk-Kyu Seoh and Debby Walthall

Administrator Contact: 404-413-5379 or 404-413-5363

Application: The Typhoon 9400 is an older version fo the TyphoonTrio, and can image store phosphor autoradiography and many types of fluorescent stains (direct blue-excited fluorescence – 457 and 488 nm; direct green-excited fluorescence – 532 nm; direct red-excited fluorescence – 633 nm. This imager is specifically designed for 2-D DIGE multicolor scanning and detection of CyDye DIGE Fluor dye-labeled proteins. Other common fluorescent stains that this imagers can image are Deep Purple, Coomasie Blue, EtBr, SYPRO red and SYPRO Ruby. They can image agarose and acrylamide gels, blots, microarray slides and imaging plates of many different sizes.

Make: GE Healthcare

Form/Manual: Typhoon 9010 Phospho/ Fluorescent

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Alpha Innotech (Bucher)

Location: Kell 405

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Key RSDP Features of FluorChem Imaging System

• Resolution: Megapixel CCD resolution: 1.92 Megapixels
• Proprietary algorithms for 3D image contour, image sharpening, and noise reduction for improved image resolution and quality.
• Sensitivity: Deep CCD peltier cooling facilitates multiple hour long exposures and exceptional image quality.
• FluorChem as an optimized for CCD imaging technology to generate femtogram sensitivity levels.
• Dynamic Range: >3.0 O.D. dynamic range with FluorChem Imaging System.
• Performance: Fast, easy, versatile, and powerful for comprehensive fluorescence and chemiluminescence applications AND basic gel documentation.
• Automatic lane/band detection and analysis with smiling corrections are easily done with a single button click!
• Capture grayscale or true color images with automatic RGB color image overlay feature.

Make: Bucher biotec

Form/Manual: Alpha Innotech Imaging Systems Manual

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Incubator

Location:PSC 555, NSC 368, Kell Hall 405

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Culture and Cell Incubation

Make: Thermo-Fisher

Form/Manual: Incubator

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Shaker, C25 Floor

Location: NSC 368

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The classic C25 incubator shakers are large capacity shakers utilizing an eccentric counter balanced drive to provide horizontal plane rotary motion in a 1" (2.54 cm) circular orbit. Shaker speed and temperature are precisely regulated by a microprocessor controller that maintains setpoints within very rigid tolerances. Touch the keypad to select and display temperatures from a few degrees above ambient to 60º (Model C25), and 17º below ambient (minimum +4º) to 60º, (Model C25KC).

Make: New Brunswick

Form/Manual: Shaker, C25 Floor

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Shaker, Series 25 Floor

Location: NSC 368,

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The Series 25 incubator shakers are medium to large capacity shakers whose shaker speed and temperature are precisely regulated by a microprocessor controller that maintains setpoints within very rigid tolerances. Touch the keypad to select and display temperatures from a few degrees above ambient to 60º (Model Series 25), and 17º below ambient (minimum +4º) to 60º, (Model Series 25KC). NB. Even though this particular Shaker is well able to regulate it's temperature it NEEDS an additional thermometer to view temperature within the cabinet

Make: New Brunswick

Form/Manual: Shaker, Series 25 Floor

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Shaker, E25 Floor

Location: PSC 555

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: New Brunswick Floor Incubator Shakers E25R offers the largest capacity (flasks up to 6 L) in the Excella® line for shaking, incubating, and optional cooling. Choose from standard ambient models or refrigerated models that operate at 15°C below ambient to 60°C. The reliable microprocessor control system enables precise setting of speed, running time and temperature and automatically compensates for changes in load, voltage or temperature. - See more at: http://www.daigger.com/store/new-brunswick-e25-floor-model-incubator-shakere25r-floor-model-incubator-shaker-with-refrigeration9819e/16499#sthash.dZtcGzpO.dpuf

Make: New Brunswick

Form/Manual: Shaker, E25 Floor

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Shaker, Classic Floor

Location: PSC 637, Kell Hall 405

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The classic incubator shakers are large capacity shakers utilizing an eccentric counter balanced drive to provide horizontal plane rotary motion in a 1" (2.54 cm) circular orbit. Shaker speed and temperature are precisely regulated by a microprocessor controller that maintains setpoints within very rigid tolerances. Touch the keypad to select and display temperatures from a few degrees above ambient to 60º (Model C25), and 17º below ambient (minimum +4º) to 60º, (Model C25KC).

Make: New Brunswick

Form/Manual: Shaker, Classic Floor

labcommander
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Shaker, MaxQ5000 Floor/Refrig

Location: PSC 555, PSC 639

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application:Thermo Scientific™ MaxQ 5000 shakers are versatile incubated/refrigerated units featuring a easy side access drain system making cleaning spills easy and retractable foot casters to easily position the shaker in the lab. MaxQ 5000 incubated and refrigerated floor shakers feature a foot pedal for hands-free operation and a large slanted viewing window to check the progress. These shakers are ideal for cell culture, solubility studies, plasmid purification, staining and destaining, extraction procedures, protein expression and many other applications

Make: Thermo Scientific

Form/Manual: Shaker, MaxQ5000 Floor/Refrig Service Form

labcommander
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Shaker, Innova Floor

Location: NSC 460

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Large-capacity, environmental shakers for incubation and/or refrigeration.
• Wide temperature range
• Uniform temperature, regardless of sample location
• Rapid temperature equilibration

Make: New Brunswick

Form/Manual: Shaker, Innova Floor

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Stacking Shaker, Floor/Refrig

Location: PSC 555, PSC 639, NSC 368, NSC 460, STA, Turner Suite Rm #2

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application: Thermo Scientific™ MaxQ 6000 shakers offer sample storage and orbital shaking with intuitive, easy-to-use digital controls.

Make: Thermo Scientific

Form/Manual: Stacking Shaker Floor/Refrig

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Stacking Shaker, Floor

Location: PSC 555, PSC 833, NSC 368,

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application: Thermo Scientific™ MaxQ 6000 shakers offer sample storage and orbital shaking with intuitive, easy-to-use digital controls.

Make: Thermo Scientific

Form/Manual: Stacking Shaker Floor

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Shaker C24/25 Tabletop Shaker

Location: PSC 637

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application: Features a heavy-duty drive and cool-running motor for long life and quiet operation under heavier loads and higher speeds.
Intuitive, spill-proof keypad allows easy input of parameters
0.56 in. (1.4cm) LED display shows running conditions and diagnostics (all models except E1)
UniCentric™ drive and brushless DC motor ensure quiet, vibration-free operation
Versatile—use same accessories as other New Brunswick Scientific shakers
Two-year warranty on parts and labor (US and Canada only)

Make: New Brunswick

Form/Manual: Shaker C24 Tabletop

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Shaker E24/E25 Tabletop Shaker

Location: PSC 555, NSC 460, Kell Hall 405

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application:The E24 / E25 shakers combine incubated/refrigerated temperatures with orbital shaking in a compact footprint. They use minimal bench space and are affordably priced. These benchtop shakers are ideal for cell culture, solubility studies, extraction procedures, protein expression and many other applications

Make: New Brunswick

Form/Manual: Shaker E24 Tabletop

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Shaker MaxQ400 Tabletop Shaker

Location: ;PSC 555

Administrator: Sonja Young and Debby Walthall

Administrator Contact: (404) 413-5363

Application: Thermo Scientific™ MaxQ400 is a versatile, incubated and refrigerated shaker ideal for incubating a small number of vessels.
The MaxQ400 shakers combine incubated/refrigerated temperatures with orbital shaking in a compact footprint. They use minimal bench space and are affordably priced. These benchtop shakers are ideal for cell culture, solubility studies, extraction procedures, protein expression and many other applications

Make: Thermo Scientific

Form/Manual: Shaker MaxQ400 Tabletop

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Mini Q4450 Shaker Tabletop

Location: PSC 637, NSC 340, NSC 488

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Thermo Scientific™ Mini 4450 is a versatile, incubated and refrigerated shaker ideal for incubating a small number of vessels. The Mini4450 shakers combine incubated/refrigerated temperatures with orbital shaking in a compact footprint. They use minimal bench space and are affordably priced. These benchtop shakers are ideal for small cell culture, solubility studies, extraction procedures, protein expression and many other applications

Make: Thermo Scientific

Form/Manual: Mini Q4450 Shaker Tabletop

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Rotating Shaker MaxQ2508

Location: PSC 535, PSC 659B, NSC 338, NSC 448

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Open air benchtop shakers are ideal for small cell culture, solubility studies, extraction procedures, protein expression and many other applications

Make: Thermo Scientific

Form/Manual: Rotating Shaker MaxQ2508

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Oven Tabletop

Location:PSC 555, PSC 645, NSC 484 (Precision and Griev)

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Sterilize glassware and other high temperatre needs

Make: Thermo-Fisher

Form/Manual: Oven Tabletop

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Furnace

Location: PSC 545

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Thermo-Fisher chamber furnaces are ideal for use in crowded laboratories and for common applications such as materials testing, burning, rapid heat processes.

Make: Thermo-Fisher

Form/Manual:

labcommander
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AFM Multimode VIII

Location: PSC 661

Administrator: Gemeia Cameron or John Houghton

Administrator Contact: (404) 413-5363 or (404) 413-5390

Application: The AFM is one of the foremost tools for imaging, measuring, and manipulating matter at the nanoscale. The information is gathered by "scanning" the surface on which the biological samples is based with a mechanical probe. Piezoelectric elements that facilitate tiny but accurate and precise movements on (electronic) command enable the very precise scanning. Electric potentials can also be scanned using conducting cantilevers. In this more advanced versions, currents can be passed through the tip to probe the electrical conductivity or transport of the underlying surface. There are various modes of scanning, which include:
•Contact Mode
•Non-Contact Mode
•Peak Force Mode (new to the VIII upgrade) which analyzes each tip-sample interaction to extract nanomechanical properties including modulus, adhesion, deformation, and dissipation.

Make: Bruker

For Use and Further Information: Atomic Force Microscope Services

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Deconvolution Microscope

Location: PSC 873

Administrator: Bill Walthall

Administrator Contact: (404) 413-5391

Application: Deconvolution is a computationally intensive image processing technique that is being increasingly utilized for improving the contrast and resolution of digital images captured in the microscope. The foundations are based upon a suite of methods that are designed to remove or reverse the blurring present in microscope images induced by the limited aperture of the objective.
This upgrade using a new UNIX based OS, allows the user to image material in 4D at high resolution (6.7 mm pixels). A CoolSnap HQ2 CCD camera allows the software to apply sophisticated algorithms to remove out of focus light and reassign refracted light to the calculated point of origin. The result is an image with a broad dynamic range of signal and stunning detail. The system is equipped with DIC optics, allowing the user to combine DIC and fluorescent light microscopy. The upgraded software is considerably more user friendly. 3D and 4D reconstructions are easier to setup and also has improved resolution. Possible applications include:
•High resolution imaging of cells and subcellular structure with multichannel detatil.
•Imaging of living cells over time.
•Detection and quantication of cell permeant, expressed, or injected fluorescent probes in living cells
•3D and 4D reconstructions

Make: DeltaVision

Form/Manual: Deltavision Website

labcommander
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TILL Photonics Imaging system

Location: Science Annex Rm 500

Administrator: Gemeia Cameron or Liana Artinian

Administrator Contact: (404) 413-5363 or (404) 413-5345

Application: TILL Photonics Imaging system offers advanced equipment used for a variety of different applications studying dynamic processes in living cells such as:

•Measurement of rapidly changing intracellular ion concentrations (Ca2+, Na+ and other ions) e.g. in neurons.
•Study of signal transduction processes in response to e.g. growth factors or cytokines.
•Study of calcium sensitive processes.
•Simultaneous imaging of different target molecules within one sample using multiple staining with different dyes or probes.
•Metabolic transport.
•Cell-cell interactions.

Make: TILL Photonics

Form/Manual: TILL Photonics Imaging System Service Form

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Laser Capture System

Location: PSC 633

Administrator: Sonja Young

Administrator Contact: (404) 413-5363 or (404) 413-5345

Application: The Arturus Pixcell IIE laser capture microdissection system is specifcally designed around an olympus microscope for the isolation of specific cell types or tissue from tissues processed by paraffin embedding or cryo-sectioning. This microdisections permits the molecular analysis of pure populations of cells or specific regions of tissures. The system has a number of applications,

•  Analysis of cell surface antigens and biomarkers
•  Immunophenotyping of cells for stem cell and hematopoietic cell lineage studies
•  Hematology
•  Cell size and Cell Cycle studies
•  Apoptosis, necrosis and live/dead cell assays

Make: Life Technologies (Arcturus)

Form/Manual: System Brochure

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Keyence Fluorescent Microscope

Location: PSC Rm 535

Administrator: Gemeia Rice

Administrator Contact: (404) 413-5363

Application: An automated all-in-one fluorescence microscope that offers similar functionality to a confocal, live cell incubator, and slide scanner. BZ-X700 series
•Cell-cell interactions.

Make: BZ-X700 series

Form/Manual: Keyence BZ-X700 series microscope Service Form

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Zeiss Axioimager 2 Fluorescent Microscope

Location: PSC Rm 657

Administrator: John Houghton

Administrator Contact: (404) 413-5390

Application: Axio Imager 2 combines everything you've ever asked for in your research environment: brilliant optics and bright fluorescence. The contrast manager and light manager ensure defined conditions and reproducible results at all times. Use ACR to automatically detect and configure objectives and filter sets for Axio Imager.Z2

Make: Zeiss Axioimager 2

Form/Manual: Zeiss Axioimager 2

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Zeiss Axio Observer 2 Fluor. Microscope

Location: PSC Rm 657

Administrator: John Houghton

Administrator Contact: (404) 413-5390

Application: The Axio Observer 2 inverted, automated fluorescent Microscope combines everything you've ever asked for in your research environment: brilliant optics and bright fluorescence. The contrast manager and light manager ensure defined conditions and reproducible results at all times. Use ACR to automatically detect and configure objectives and filter sets for Axio Observer.Z2

Make: Zeiss Axio Observer 2

Form/Manual: Zeiss Axioimager 2

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Biomek UV/Vis Plate Reader

Location: PSC 559

Administrator: Gemeia Cameron and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: n/a

Make: Beckman

Form/Manual: Biomek Plate Reader UV/Vis Service Form

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Enspire with despenser UV/Vis/Fluorescent Plate Reader

Location: PSC 559

Administrator: Gemeia Cameron and Hyuk Kyu Seoh

Administrator Contact: 404-413-5379

Application: You can access pre-stored calculations such as ratios which provide double emission readout in a FRET-based or cell viability assay. You can run endpoint assays with one or two wavelengths or scan the entire wavelength range. You can also follow enzyme kinetics in real time.

Other applications: Enzyme kinetics - Temperature controlled reagent reservoirs with plate temperature control for stability and accuracy. Can be integrated with PerkinElmer automated liquid handling platforms; Cellular - Well area scanning. Can be integrated with PerkinElmer automated liquid handling platforms; Viable cell - Optional bottom reading offering signicant improvements for viable cell assays; ELISA - Photometric technology and AlphaLISA allow conversion to homogenous ELISA technology; GFP and other fluorescent proteins - Quad monochromators, bottom reading and well scanning; Luminescent Reporter gene and cell growth - Ultra-sensitive luminescence with dynamic range for low transfection rates; Calcium - dispenser for suspension cells convenient in receptor activation studies; Dual luciferase flash - enabled by dispenser option; Nucleis acid/protein quantitation - photometric technology.

Make: Perkin Elmer

Form/Manual:

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MD Plate Reader UV/Vis; filterless

labgate
Location: PSC 559, STA: Turner Suite, Rm #2

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: n/a

Make: Molecular Dynamics

Form/Manual: MD Plate Reader UV/Vis; filterless Service Form

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Victor with dispenserPlate Reader UV/Vis/Fluorescent

labgate
Location: PSC 633, NSC 338A

Administrator: Debby Walthall

Administrator Contact: 404-413-5363

Application: VICTOR3 comes with several standard features such as temperature control, shaking, area scanning, adjustable measuring height, below fluorescence reading, dual label/ratio reading and kinetics. A dispenser is also available for cell-based assays by enabling fast kinetics measurements, enzyme assays and numerous other cellbased drug discovery assays.

Technologies and reading modes- Fluorescence Intensity (340-850* nm)

• Fluorescence Polarization (400-850* nm)
• Time-Resolved Fluorescence (TRF)
• Luminescence
• Glow, flash and dual luminescence
• Absorbance (340-800 nm)
• UV Absorbance (260 nm/280 nm)

Make: PE

Form/Manual: Victor Plate Reader UV/Vis/Fluorescent

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MALDI ToF-Tof

Location: PSC 537A

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application:

•Powerful RDA features of GPS Explorer™ Software for flexible acquisition and data processing.
• Unmatched sensitivity (subfemtomole) in both MS and MS/SM modes
• Enhanced Precursor Ion Selection
• Profile biomolecules directly on tissue samples; sample (tissue section, whole organs or whole animals) can be analyzed directly, no extraction of compounds is required and no sample labeling required.
•ProteinPilot Software 2.0 enables searching for over 100 post-translational modifications, up to 45 work-up modifications and all amino acid substitutions, genetic variants and unexpected cleavages.
• Enhanced Mass accuracy. Using an internal standard, 2.5 ppm mass accuracy is achieved, increasing confidence in protein ID.
• Quantitation of Specific proteins using iTRAQ reagents. iTRAQ reagents are multiplexed sets of 8 isobaric reagents which allows for sophisticated time course studies, absolute quantitation and monitors qualitative protein changes under different physiological conditions.
• Biomarker Discovery using iTRAQ reagents. Besides biomarker identification, iTRAQ allows quantitation that would indicate which proteins are changing in response to a given environmental change, drug treatment or disease.
• Post-source-decay for peptide sequencing. MALDI CID capability for protein sequence determination and structural analysis.

Make: ABI

For Use and Further Information: MALDI / Proteomic Services

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2D Gel Electrophoresis

Location: PSC 537A

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:This technique sorts proteins according to two independent properties in 2 steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the second-dimension step, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), separates proteins according to the molecular weights (MW). Each spot on the resulting two-dimensional array corresponds to a single protein species in the sample.

Make: GE Healthcare

Form/Manual: 2D Gel Electrophoresis

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Typhoon Trio Imager

labgate
Location: PSC 537

Administrator: Hyuk-Kyu Seoh and Debby Walthall

Administrator Contact: 404-413-5379 or 404-413-5363

Application: The Typhoon Trio can image Storage phosphor autoradiography and many types of fluorescent stains (direct blue-excited fluorescence – 457 and 488 nm; direct green-excited fluorescence – 532 nm; direct red-excited fluorescence – 633 nm. This imager is specifically designed for 2-D DIGE multicolor scanning and detection of CyDye DIGE Fluor dye-labeled proteins. Other common fluorescent stains that this imagers can image are Deep Purple, Coomasie Blue, EtBr, SYPRO red and SYPRO Ruby. They can image agarose and acrylamide gels, blots, microarray slides and imaging plates of many different sizes.

Make: GE Healthcare

Form/Manual: Typhoon Trio Phospho, Fluorescent

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Spot Picker

Location: PSC 537

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Designed for use with most common 2-D gels. A protein pick-list is generated form 2D PAGE Gel, either automatically using predefined selection criteria, or manually. The protein pick-list is transferred to Ettan™ Spot Picker, which then automatically picks the protein spots according to the list. A wash step is performed after each picking session, and the location of each picked protein in its respective microplate well is automatically logged to facilitate sample tracking. When picking a limited number of protein spots, or for pooling of samples, the software also includes a “click-and-pick” function whereby proteins can be picked without a pick-list. The system accommodates any gel size up to 250 - 280 mm and picks from 1-mm as well as 1.5-mm thick gels. respectively. Processing of 96 samples is performed in less than 30 min.

Make: GE Healthcare

Form/Manual: Spot Picker

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Digester

Location: PSC 537

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Digester is a highly versatile instrument designed to perform in-gel digestion of proteins captured in 2-D gel electrophoresis spots. The instrument acts directly on Ettan™ Spot Picker output files, automatically tracking samples from spot picking to completed digestion using simple "plug-and-play" routines. It allows robust and reproducible digestion, sensitive to 100 fmol range. Default digestion protocols are optimized and compatible with Coomassie™, silver, and fluorescence staining systems. A method editor also allows customization of methods. Standard microplates further increase ease-of-use, as do low-salt digestion buffers, eliminating the need for desalting (no need for C18 clean-up)

Make: GE Healthcare

Form/Manual: Digester Service Form

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7500 Fast

labgate
Location: PSC 535, PSC 633, NSC 338

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: The 7500 Real-Time PCR Systems offer high performance, multicolor real-time PCR wherever your research takes you. Choose from our Fast and standard 7500 systems.

Real-time quantitative PCR has many applications including the precise measurement of changes in gene expression, pathogen detection, and post-PCR detection in allelic discrimination (SNPs) assays. It is also ideal for plus/minus assays to confirm the presence or absence of a pathogen, transgene, or other specific target sequence:

• 96-well-format high-speed thermal cycling, delivering high quality results in less than 30 minutes
• Offers robust high resolution melting analysis
• Learn more about the 7500 Fast Real-Time PCR System

Make: Life Technologies

Form/Manual: 7500 Fast Operation Manual

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StepOne

labgate
Location: PSC 535

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: StepOnePlus™ Real-Time PCR Systems offer intuitive software, Fast (<40 min) and standard (<2 hr) runs, and are configured for PC-free or networked operation. These factory-calibrated systems make it easy to step up to high-performance qPCR. And now these systems support high resolution melt (HRM) software

Make: Life Technologies

Form/Manual: StepOne Operation Manual

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Biomek NX

Location: PSC 533

Administrator: Ping Jiang

Administrator Contact: 404-413-5370

Application: Biomek NX puts every aspect of liquid handling – including pipetting, dilution, dispensing, and integrations – into a single, automated system that is powerful and flexible. The NXP workstation offers multiple configurations, including multichannel, Biomek NX features Span-8 with gripper and can rotate a full 360°. The Span 8 is used for rapid plate-to-plate; tube to plate applications and assay miniaturization. Pipetting performance for low-volume reaction setup and assay miniaturization, as well as accurate and repeatable results extending into the submicroliter range. Applications

• DNA/RNA purification
• PCR setup and cleanup
• DNA Sequencing setup and cleanup
• Reproducible and high throughput for mutliple assays

Make: Beckman

Form/Manual: Biomek NX

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Biomek 2000

Location: PSC 559 -storage

Administrator: Debby Walthall

Administrator Contact: 404-413-5363

Application: The Biomek 2000 Laboratory Automated Workstation provides simple, intelligent automation of liquid-handling tasks. Pipetting, diluting and dispensing operations are performed quickly, easily and automatically. The modular platform allows expansion of system capability to include plate heating and cooling, plate washing, high-density transfers, photometric measurement.

These applications include:

Immunology: ELISA, EIA, RIA, Forensic Blood Typing.

Molecular Biology: DNA Sequencing, DNA Fingerprinting, PCR Processes, DNA Template Preparation, High-Density Library Maintenance and Screening, Sample Quantitation and Normalization, as well as Protein/enzymes Assays

Make: Life Technologies

Form/Manual: Biomek 2000

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LS6500 Scintillation Counter

Location: PSC 555, 659, NSC 488, Kell Hall 405

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The LS 6500 can count 3H, 14C, 125I, 35S and 32P. The instrument calculates counts per minute (cpm). Data can be normalized and blanks and/or backgrounds can be subtracted. You can set up a manual count or set up a User program with your specific needs (isotope and count time, for example). (LS6500 Operator’s Manual).

Make: Beckman

Form/Manual: LS6500 Scintillation Counter

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1480 Wizard Gamma

Location: PSC 555 NSC 488

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: 30 mm of solid lead provides effective shielding which gives constant background and minimal crosstalk.
Multichannel analyer (MCA) technology incorporates a high resolution 1024-channel multichannel analyzer dedicated to each detector. 
It can count manually as well as automatically.
With an energy range up to 900 keV and a powerful set of software, Wizard gives you results from a wide range of applications including all RIA and IRMA tests, screening assays, etc. 
Ideal for chromium release studies - no crosstalk from samples on the conveyer means that the Wizard is ideal for working with higher energy isotopes such as Chromium-51; Vendor: Wallac

Make: Perkin Elmer

Form/Manual: 1480 Wizard Gamma

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Frontier FTIR

Location: PSC 543

Administrator: Hyuk-Kyu-Seoh and Gemeia Cameron

Administrator Contact: (404) 413-5363

Application: The Frontier FTIR is powerful and adaptable range of Frontier™ IR systems and is assured to meet the needs of your current IR analysis. It can also be expanded to offer flexible system configurations in the near, near-mid, mid-far and far infrared regions, Frontier can support an extensive range of applications thanks to its superb sensitivity and configurability.
Chemical and Materials: From troubleshooting manufacturing problems, identifying product contaminants to gaining a deeper understanding of advanced material properties.

Research & Academia: Complete far-IR characterization of synthesized and novel materials, or quickly adapt the flexible platform for different research areas or groups

Make: Perkin Elmer

Form/Manual: Frontier_FTIR Brochure

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Biophotometer

Location: Coming in 2013

Administrator: Ping Jiang and Gemeia Cameron

Administrator Contact: (404) 413-5363

Application: The Eppendorf Biophotometer® is a small, very compact spectrophotometer for measurements in the UV and VIS range. Spectra can be recorded and individual wavelength measurements can be made in a spectral range of 200 nm to 830 nm. What's more, the cuvette shaft of the Biophotometer kinetic is temperature controlled from +20°C to +42°C, meaning that enzyme and substrate kinetics can be measured directly in the device. No device upgrade with accessories is necessary for temperature control.The Biophotometer fluorescence in addition to the Biophotometer basic has the option to determine very low concentrations of biomolecules using fluorescent dyes and can increase the measuring range by a factor of 1,000, for detecting DNA for example.

Product characteristics:
• UV/Vis spectral range 220 nm to 830 nm
• Xenon flash lamp with very long service life
• Preprogrammed methods for a quick start
• Guided software process to minimize errors

Make: Eppendorf

Form/Manual: Biophotometer Operation Manual

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Biophotometer

Location: PSC 545, Kell 405

Administrator: Ping Jiang and Gemeia Cameron

Administrator Contact: (404) 413-5363

Application: The BioPhotometer plus offers a compact UV/Vis photometer for use in molecular biology, biochemistry and cell biology and provides instant, out-of-the-box access to 32 routine methods, of which 9 methods are freely programmable,

e. g.:
• Measurement of DNA, RNA and protein concentration (UV and colorimetric)
• Incorporation rate of fluorescent molecules (550 nm/650 nm), e. g., for microarray experiments
• Enzymatic assays (e.g., peroxidase, alkaline phosphatase, β-galactosidase)
• Optical density of cells (OD 600)

Both measurement and calculation of results are performed at the press of a button, and the results of the analysis, as well as all accompanying data, are available at a glance. This guarantees safe, error-free operation and reliable results.

Make: Eppendorf

Form/Manual: Biophotometer Operation Manual

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Nanodrop

Location: PSC 533, NSC 338

Administrator: Ping Jiang and Gemeia Cameron

Administrator Contact: (404) 413-5370 and (404) 413-5363

Application: n/a

Make: Thermo/Fisher

Form/Manual: Nanodrop Operation Manual

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Nanophotometer P360

Location: PSC 633

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5363

Application: The Nanophotometer is an All-in-One spectrophotomer with micro volume (0.3 ul) and optimized for quantification and purity assessment of nucleic acid and protein samples. Sample Compression Technology allows for accurate (+/- 5%) and reproducible (.005 Abs) readings of nucleic acids, proteins and peptides. This technology also is tolerant of a wide range of buffers that often interfere with an accurate reading. Funding provided by Tech fee.

Make: Implen

Form/Manual: Nanophotometer Operation Manual

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Nanovue

Location:PSC 535, STA, Turners Suite, Rm# 2

Administrator: Ping Jiang and Gemeia Cameron

Administrator Contact: (404) 413-5363

Application: The NanoVue spectrophotometer is an easy-to-use and reliable instrument for the measurement of nucleic acid and protein samples. Samples of 0.5 μl to 5 μl can be pipetted directly onto the gold-colored sample plate for measurement, and then simply recovered using a pipette. If sample recovery is not required, the sample plate can be quickly and easily wiped clean.

Make: GE Healthcare

Form/Manual: Nanovue Operation Manual

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Mastercycler Gradient

Location: NSC 460

Administrator: Debby Walthall and Sonja Young

Administrator Contact: (404) 413-5363

Application: The Mastercycler® family offers ultimate reliability combined with easy to use software. The universal block can work with all common consumables and increases the range of application flexibilities. The Mastercycler gradient has SteadySlope® technology ensuring optimal, constant heating and cooling rates, which means that results of gradient experiments can be transferred to routine applications.

Features:
• SteadySlope® and Triple Circuit Technology
•  Gradient block1: up to 20°C gradient span is possible at any step of the PCR program
•  Universal block2: Possible to use 0.2 mL tubes, 0.5 mL tubes or plates
•  Simple programming for gradient function, adjustable ramp rates, time and temperature increments, incubation mode, link function, pause function
•  Software-supported in situ Adapter
•  RS-232 and printer interface
•  Auto-restart option in the event of a power failure
personal card system

Make: Eppendorf

Form/Manual: Mastercycler Gradient Service Form

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Mastercycler Gradient Pro

Location: PSC 545, PSC 659, NSC 338, NSC 368, NSC 488

Administrator: Debby Walthall and Sonja Young

Administrator Contact: (404) 413-5363

Application: The Mastercycler® family offers ultimate reliability combined with easy to use software. The universal block can work with all common consumables and increases the range of application flexibilities. The Mastercycler gradient has SteadySlope® technology ensuring optimal, constant heating and cooling rates, which means that results of gradient experiments can be transferred to routine applications.

• Advantages of Mastercycler pro:
• Ultimate reduction of evaporation
• Extremely fast heating and cooling rates
•  Gradient blocks with SteadySlope technology
•  Intuitive graphic programming
•  Display to indicate cycler number in a network
•  Optional self-test of peltier elements

Make: Eppendorf

Form/Manual: Mastercycler Gradient Pro Service Form

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Optima XL-100K Ultra Floor

Location: PSC 543, PSC 637, PSC 659, NSC 340, NSC 460, STA: Turner Suite, Rm #2

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Some of the separation Methods used in preparative ultracentrifugation:

Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima XL-100K Ultra Floor/a>

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Optima MAX Ultra Tabletop

Location: PSC 555

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima MAX Ultra Tabletop

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Optima MAX-XP Ultra Tabletop

Location: PSC 555

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima MAX-XP Ultra Tabletop

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Optima TL Tabletop

Location: PSC 545, PSC 659, NSC 460, STA, Turner Suite Rm #2

Administrator: Sonja Young

Administrator Contact:(404) 413-5363

Application: Some of the separation Methods used in preparative ultracentrifugation:

Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima TL Tabletop

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Optima TLX Tabletop

Location: PSC 543, NSC 340

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima TLX Tabletop

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UV Crosslinker

Location: PSC 955, NSC 338

Administrator: Debby Walthall

Administrator Contact: 404-413-5363

Application: The Stratagene UV crosslinker is designed to crosslink DNA or RNA to Nylon, nitrocellulose, or nylon-reinforced mitrocellulose membranes after Northern or Southern blots, dot blotting and colony or plaque lifts. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80 °C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. Other applications include nicking ethidium bromide stained DNA in agarose gels and gene mapping ofr creating cleavage-inhibiting thymine dimers.

Make: Fisher

Form/Manual: UV Crosslinker

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Milli-Q

Location: PSC 535, PSC 537, PSC 662, PSC 657, NSC 338A and NSC 448

Administrator: Debby Walthall

Administrator Contact: 404-413-5363

Application: The Milli Q has a 3 step purification process that produces water with extremely low ionic, organic, pyrogen and nuclease contamination; suitable for PCR, 2-D electrophoresis, DNA sequencing, cell culture and RNA applications. 
• Designed to specifically target contaminants detrimental to particular applications such as HPLC, molecular biology and ICP-MS.
• The systems incorporate a high precision resistivity monitor that records conductivity or resistivity (compensated or not at 25 °C), and also feature a built-in TOC monitor with a 1-999 ppb detection range for accurate analysis of organics. 
• Milli-Q resistivity and TOC meters are designed to take into account the suitability test requirements of USP 28-NF21.
• Includes a Pyrogard 5000 ultrafiltration cartridge for the production of pyrogen- and nuclease-free ultrapure water (<0.001 EU/mL), particularly important for life science or molecular biology applications. 
• The ultraviolet photo-oxidation process (UV at 254 and 185 nm) can effectively carry out the destruction of organic molecules, to reduce TOC levels to < 5 ppb, improving consistency and sensitivity in applications such as HPLC, IC, solid-phase extraction and UV spectroscopy. produces water with extremely low ionic, organic, pyrogen and nuclease contamination; suitable for PCR, 2-D electrophoresis, DNA sequencing, cell culture and RNA applications.

Make: Millipore

Form: Milli-Q Service Form

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