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Jesse Gardner
Lab / Office: PSC 533 / 521
Tel: 404-413-5370DNA Sequencing Services
Cell, Protein and DNA Core Facilities
About DNA Sequencing Services
Our mission is to improve productivity and advance your research progress. CPD Core Facilities provide reliable DNA Sequencing services to researchers at GSU and other academic, pharmaceutical, clinical institutions as well as governmental agencies. Our focus is to porvide excellent scientific service at competitive prices. We have 3730 Genetic Analyzers (Applied Biosystems) are the instrumental backbone of the facility; each providing an automated capillary electrophoresis system that can separate, detect and analyze up to 48 capillaries of fluorescently labelled DNA fragments in 1 run, with the potential to multiple run within a given 24 hr period. The 3730 Genetic Analyzer analyzes DNA through sequence and fragment analysis.Service TypesThe DNA facility offers three service options for submitting your sequencing order: PCR Prep/Cleanup, Basic Sample Prep, and Premix Sample Prep. Please follow the sample submission guidelines to prepare your DNA for sequencing. Data is reported within 2-3 business days after samples are received. When submitting samples for analyis we ask that you provide sufficient DNA/primer for two sequencing reactions. This allows us to repeat a sample in case of technical failure.
Please follow the links below to view the submission guidelines:
PCR Prep/Cleanup
Basic Sample Prep
Premix Sample Prep
DNA Service 1
$7.00 /sequence rxnOption available for Plasmid DNA and RNA Products.
DNA Service 2
$30.00 /sequence rxnOption available for BAC DNA, Cosmid DNA preparation.
DNA / RNA Gen- Transcript- ome
Call for PricingOption available for Genomic RNA Sequences
DNA / RNA Gen- Transcript- ome
Call for PricingOption available for Genomic RNA Sequences
How to Request Service
Complete the service request form and submit the form to Jesse Gardner. Prepare your samples according to our submission guidelines for best results. Please note that forms will not be accepted without an account number and PI signature. If you have any questions, feel free to contact Jesse Gardner by phone or by email at jgardner26@gsu.edu.
DNA Sequencing Basic Form
DNA Sequencing Premix FormAdditional Links
Provided below are addtional links to software, primer selection guidelines, primers in stock and trouble shooting that can be utilized to analyze or assist you with your results if they did not return as expected.
Software
Software to view chromatogram. Several choices are available through Chromas, Four Peaks and LaserGene.
Chromas Lite LaserGene
Chromas 2.31 Four Peaks
Chromas Pro
Primers
Primer Selection Guidelines
Primers in Stock
Support
Troubleshooting your results
