Cell Protein DNA Core Facility

gemeia

Ping Jiang
Lab / Office: PSC 533 / 521
Tel: 404-413-5370

DNA Sequencing Services
Cell, Protein and DNA Core Facilities

About DNA Sequencing Services

Our mission is to improve productivity and advance your research progress. CPD Core Facilities provide reliable DNA Sequencing services to researchers at GSU and other academic, pharmaceutical, clinical institutions as well as governmental agencies. Our focus is to porvide excellent scientific service at competitive prices. We have 3730 Genetic Analyzers (Applied Biosystems) are the instrumental backbone of the facility; each providing an automated capillary electrophoresis system that can separate, detect and analyze up to 48 capillaries of fluorescently labelled DNA fragments in 1 run, with the potential to multiple run within a given 24 hr period. The 3730 Genetic Analyzer analyzes DNA through sequence and fragment analysis.

Service Types

The DNA facility offers three service options for submitting your sequencing order: PCR Prep/Cleanup, Basic Sample Prep, and Premix Sample Prep. Please follow the sample submission guidelines to prepare your DNA for sequencing. Data is reported within 2-3 business days after samples are received. When submitting samples for analyis we ask that you provide sufficient DNA/primer for two sequencing reactions. This allows us to repeat a sample in case of technical failure.

Please follow the links below to view the submission guidelines:
PCR Prep/Cleanup
Basic Sample Prep
Premix Sample Prep

DNA Service 1

$6.50 / sequence rxn

Option available for Plasmid DNA and RNA Products.

DNA Service 2

$30.00 / sequence rxn

Option available for BAC DNA, Cosmid DNA preparation.

Genomic DNA
Small / Ion-PGM

Call for Pricing

Option available for Genomic Sequence (Prokaryote, Virus)

Genomic DNA
Large / Ion-Proton

Call for Pricing

Option available for Genomic Sequence (Yeast, Human etc.)

How to Request Service

Complete the service request form and submit the form to Ping Jiang. Prepare your samples according to our submission guidelines for best results. Please note that forms will not be accepted without an account number and PI signature. If you have any questions, feel free to contact Ping Jiang by phone or by email at pjiang@gsu.edu.

DNA Sequencing Basic Form
DNA Sequencing Premix Form

Additional Links

Provided below are addtional links to software, primer selection guidelines, primers in stock and trouble shooting that can be utilized to analyze or assist you with your results if they did not return as expected.

Software
Software to view chromatogram. Several choices are available through Chromas, Four Peaks and LaserGene.

Chromas Lite LaserGene
Chromas 2.31 Four Peaks
Chromas Pro

Primers
Primer Selection Guidelines
Primers in Stock

Support
Troubleshooting your results

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