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Debby Walthall
Lab / Office: PSC 535 / 519
Tel: 404-413-5363

Flow Cytometry and Cell Sorting Services
Cell, Protein and DNA Core Facilities

 

About Flow Cytometry and Cell Sorting Services

Flow cytometry uses the physical characteristics of a cell as well as fluorescent reagents/markers to answer phenotypic, biochemical and molecular questions.  Cell sorting uses these same characteristics and fluorescent markers to separate specific cells of interest from a complex mix of cells.   Cell Sorting can be used for various reasons such as very high purity (95% - 99%) populations, enrichment of populations or separation of low-density populations.
Basic Costs

Accuri C6

$20 / hour

Option available for most Cytometric Analyses.

FACSCanto II

$20.00 / hour
$5.00 / set up

Option available for most Cytometric Analyses.

LRS Fortessa

$25.00 / hour
$5.00 / set up

Option available for most Cytometric Analyses.

FACS Aria II

$100.00 / hour
Service Only

Please Consult with Debby Walthall for additional options.

Service

When preparing your samples, please follow the sample submission guidelines that will be given to you by Debby. Data is reported within 2-3 business days after samples are received, or within a reaonable, mutually agreed time thereafter. Follow the links below to view the service/training guidelines:

Training on the Accuri C6
Training on the LSRFortessa II and FACSCanto II
Cell Sorting on the FACS Aria II is through SERVICE ONLY
-the FACS Aria II is only run by Debby. Researchers will prepare the samples and may assist with gating parameters. Unused sample and sorted cells will be returned to the researcher upon completion of the sort.

How to Request Service

Contact Debby Walthall by email to begin the training process on the Accuri C6, LSR Fortessa or FACSCanto II.

If you are requesting Cell Sorting services, please contact Debby Walthall by email to discuss your needs and the experiment protocol. Please contact Debby at least 2 weeks before the actual sort to ensure that there are no scheduling conflicts as sorting cell is a tie-sensitive, labor intensive process. Sorting can be done with 2 day notice, but only if the FACSARIA II and Debby are available. If you have any questions about the service or pricing, feel free to contact Debby Walthall by phone or by email at dwalthall@gsu.edu


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