gemeia

 

Hyuk-Kyu Seoh
Lab / Office: PSC 537 / 521
Tel: 404-413-5379

MALDI / Proteomic Services
Cell, Protein and DNA Core Facilities

 

About MALDI Services

MALDI mass spectrometry provides analysis of non-volatile, soluble, and insoluble analytes. MALDI (Matrix Assisted Laser Desorption Ionization) is useful for samples such as biomolecules (proteins, sugars, peptides) and large organic molecules (polymers, dendrimers). MALDI mass spectrometry applications include molecular weight determination, molecular weight distribution, proteomics, drug discovery and more. Samples analyzed include polymers, lipids, oligosaccharides, phosphopeptides, bacteria, and small molecules. In addition to providing MALDI ToF ToF analysis the Proteomics core is also able to provide additional protein services ranging from protein isolation and purification through acrylamide gels using a state-of-the-art gel spot picker, as well as protein enzyme digestion. Moreover, we are always readily available to consult with faculy, students and staff on proteomic matters.
Service

Please follow the sample submission guidelines to prepare your sample. Data is reported within 3-5 business days after samples are received, or within a reaonable, mutually agreed time thereafter. Follow the links below to view the submission guidelines:

MALDI Sample Prep

MALDI MS

$25.00 / Spot
(Less than 5 samples)

Gel Pick

In-Gel Digest

Zip Tip Clean Up

Spotting

MS

MS/MS Analysis

MALDI MS

$10.00 / Spot
+ $60.00 Setup
(More than 5 samples)

Gel Pick

In-Gel Digest

Zip Tip Clean Up

Spotting

MS

MS/MS Analysis

MS Analysis

Option 1

$14.00 / Spot

MS

MS/MS Analysis

MS Analysis

Option 2

$50.00 / Analysis

Analysis

Comparison

Gel Service

$80.00 / Gel
(Small Gel Format)

2-D Gel Setup

Supplies

Scan

Analysis

Spot Picking

Cost of Staining

Gel Service

$120.00 / Gel
(Large Gel Format)

2-D Gel Setup

Supplies

Scan

Analysis

Spot Picking

Cost of Staining

Staining

$15.00 - $200.00

Per Gel

Cost of Staining

How to Request Service

Complete the service request form and submit the form to Hyuk-Kyu Seoh (Kyu). Prepare your samples according to our submission guidelines for best results. Please note that forms will not be accepted without an account number and PI signature. If you have any questions about the service or pricing, feel free to contact Hyuk-Kyu Seoh by phone or by email at hseoh@gsu.edu.

MALDI Service Request Form

Additional Links

The proteomics facility also provides access to sophisticated analysis software to enhance the researchers ability to more fully analyze their data.

This software is available either in the proteomics core facility itself or at deignated computers scattered within the PSC or NSC

Available Software includes:
• Decyder 2 Proteomic Analysis software (GE Healthcare),
• Imagemaster 2D Proteomic Analysis software,
• ImageQuant TL Gel Analysis/Quantitation software.

Ask Kyu for more details.


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