What's New

Cell, Protein and DNA Core Facilities
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  • Accuri C6

    Debby Wathall
    Very light and small, 2 lasers: blue (488nm) and red (630 nm); 4 detectors, FL1 (533/30), FL2 (585/40), FL3 (670 LP) and FL4 (375/25); an automatic plate/tube loader; simple maintenance and simple software.
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  • Simple Western

    Hyuk-Kyu Seoh
    Reinvents the entire process without gels, transfer tanks, blots or film giving researchers a complete, walk-away solution for protein detection and characterization.
    View
  • Aria Cell Sorter

    Debby Wathall
    UV laser upgrade added. This laser excites at 355 nm. One application uses Indo-1 whose properties depend on the amount of bound Ca++. The emission of Indo-1 shifts from about 475 nm without Ca++ to about 400 nm with Ca++ when excited at 350 nm. The UV laser can also be used to look at side populations in stem cells.
    View
  • Nanophotometer P360

    Gemeia Cameron
    All-in-One spectrophotomer with micro volume (0.3 ul) and optimized for quantification and purity assessment of nucleic acid and protein samples.
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  • LAS 4000 Mini

    Gemeia Cameron
    CCD camera system for sensitive, quantitative imaging of gels and blots by chemiluminescence and white epi-illumination gel documentation.
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  • TILL Real-Time Imaging System

    Gemeia Cameron
    Used for a variety of different applications studying dynamic processes in living cells.
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  • STP-120

    Debby Walthall
    Embeds the tissue in paraffin.
    View
  • Histocenter 3

    Debby Walthall
    Embeds tissue samples into paraffin blocks for microtome sectioning.
    View
  • Microtome, Model 860 Sliding

    Debby Walthall
    Section the paraffin blocks into extremely thin slices.
    View
  • Rotary Microtome

    Debby Walthall
    Section the paraffin blocks into extremely thin slices.
    View
  • HM 550 Microtome

    Debby Walthall
    Sections paraffin blocks into extremely thin slices and ensures optimal sectioning temperatures.
    View
  • Enspire Plate Reader

    Gemeia Cameron
    Reads optical biosensor label-free, fluorescence intensity, absorbance, ultra-sensitive luminescence and Alpha technologies in microplates up to 384-wells.
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  • UVP BioDoc-it®

    Sonja Young
    Combines its innovative technologies with a specialization in fluorescent and luminescent-based imaging applications in the life sciences UVP offers a wide range of BioImaging potential from basic imaging analysis to advanced multifunctional gel documentation, etc.
    View
  • AKTA Purifier

    Hyuk-Kyu Seoh
    Designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.
    View

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Accuri C-6 Blue/Red

Location: PSC 535

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: The BD Accuri™ C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable. The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. As a result, flow cytometry has become a staple of modern laboratories around the world.

Make: BD Biosciences

Form: Accuri C-6 Blue/Red Request for Service Form

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Protein Simple Simon Western

Location: PSC 559

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: Simple Western by ProteinSimple effectivley reinvents the entire Western blot process without gels, transfer tanks, blots or film - finally giving researchers a complete, walk-away solution for protein detection and characterization. All hands-on steps from sample loading and detection to quantitative data analysis are completely automated, eliminating the manual factors that can negatively impact reproducibility, quantitation, time to result and data reliability. Simon analyzes 12 samples per run in 3-5 hours! Funding provided by Tech fee.

Make: Protein Simple

Form: Protein Simple Simon Western Service Form

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Aria Cell Sorter Blue/Red/Violet/UV

Location: PSC 659B

Administrator: Debby Walthall

Administrator Contact: (404) 413-5363

Application: This benchtop system delivers high-speed sorting and multicolor analysis. The BD FACSAria instrument is the first benchtop sorter that incorporates a fixed-alignment cuvette flow cell, which minimizes startup time and improves reproducibility. This new flow cell provides superior fluorescence sensitivity and freedom from instrument maintenance and alignment. Gel coupling to the objective lens improves collection efficiency and increases sensitivity and resolution needed ofr multicolor sorting applications. Additional "Sort setup" and "sort monitoring" features in the system software makes the BD FACSAria instrument easy to use. BD FACS™ Accudrop technology is integrated into the system for quick and accurate drop-delay determination. Stream monitoring of the breakoff point and clog detection are incorporated. The system has four air-cooled lasers at 488-nm, 633-nm, 405-nm and 355-nm wavelengths. Two- and four-way bulk sorting devices for a variety of tube sizes are also available. Water Jacketed tube holders are also available to keep cells warm or cold as needed.

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

Form: Aria Cell Sorter Blue/Red/Violet/UV Service Form

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Nanophotometer P360

Location: PSC 633

Administrator: Ping Jiang and Gemeia Cameron

Administrator Contact: (404) 413-5363

Application: The Nanophotometer is an All-in-One spectrophotomer with micro volume (0.3 ul) and optimized for quantification and purity assessment of nucleic acid and protein samples. Sample Compression Technology allows for accurate (+/- 5%) and reproducible (.005 Abs) readings of nucleic acids, proteins and peptides. This technology also is tolerant of a wide range of buffers that often interfere with an accurate reading. Funding provided by Tech fee.

Make: Implen

Form: Nanophotometer Operation Manual

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LAS4000 mini Chemiluminescence

labgate
Location: PSC 563; NSC 438

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Exceptional sensitivity for quantitation of biomolecules labeled with chemiluminescent probes Supports gel documentation of Coomassie™ blue and silver-stained samples. Accurate quantitation of closely spaced bands in complex protein samples for gels and films up to 18 x 12 cm. Low noise for longer exposure times and lower background, important for precise quantitation of very weak signals. Wide linear dynamic range for precise quantitation of faint and dark bands over four orders of magnitude in the same image, avoiding time-consuming multiple film exposures. Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision and uniform quantitation. Sensitive detection of less than 40 pg of protein on a Western blot. Sensitivity may be increased by "binning" of up to 8 x 8 pixels and smoothing. Chemiluminescent and colorimetric signals can be captured in the same image without changing the lens.

Make: GE Healthcare

Form: LAS4000 mini Chemiluminescence Service Form

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TILL Photonics Imaging system

Location: Science Annex Rm 500

Administrator: Gemeia Cameron or Liana Artinian

Administrator Contact: (404) 413-5363 or (404) 413-5345

Application: TILL Photonics Imaging system offers advanced equipment used for a variety of different applications studying dynamic processes in living cells such as:

•Measurement of rapidly changing intracellular ion concentrations (Ca2+, Na+ and other ions) e.g. in neurons.
•Study of signal transduction processes in response to e.g. growth factors or cytokines.
•Study of calcium sensitive processes.
•Simultaneous imaging of different target molecules within one sample using multiple staining with different dyes or probes.
•Metabolic transport.
•Cell-cell interactions.

Make: TILL Photonics

Form: TILL Photonics Imaging System Service Form

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STP-120 Spin Processor

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: The STP-120 Spin Processor embeds the tissue in paraffin.

Make: Fisher

Form: STP-120 Spin Processor Service Form

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Histocenter 3

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: The Histocenter 3 embeds tissue samples into paraffin blocks for microtome sectioning.

Make: Shandon

Form: Histocentre 3 Service Form

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Microtome, Model 860 Sliding

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: IMEB.inc

Form: Microtome Model 86C Sliding Service Form

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Rotory Microtome

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: Unknown

Form: Rotory Microtome Service Form

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HM 550 Microtome

Location: NSC 448

Administrator: Robert Simmons and Debby Walthall

Application: The HM550 Microtome esnures that sectioning of paraffin blocks into extremely thin slices proceeds at an optimal sectioning temperature

Make: Fisher

Form: HM 550 Microtome Service Form

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Enspire UV/Vis/Fluorescenct Plate Reader

Location: PSC 563

Administrator: Gemeia Cameron and Hyuk Kyu Seoh

Administrator Contact: 404-413-5379

Application: You can access pre-stored calculations such as ratios which provide double emission readout in a FRET-based or cell viability assay. You can run endpoint assays with one or two wavelengths or scan the entire wavelength range. You can also follow enzyme kinetics in real time.

Other applications: Enzyme kinetics - Temperature controlled reagent reservoirs with plate temperature control for stability and accuracy. Can be integrated with PerkinElmer automated liquid handling platforms; Cellular - Well area scanning. Can be integrated with PerkinElmer automated liquid handling platforms; Viable cell - Optional bottom reading offering signicant improvements for viable cell assays; ELISA - Photometric technology and AlphaLISA allow conversion to homogenous ELISA technology; GFP and other fluorescent proteins - Quad monochromators, bottom reading and well scanning; Luminescent Reporter gene and cell growth - Ultra-sensitive luminescence with dynamic range for low transfection rates; Calcium - dispenser for suspension cells convenient in receptor activation studies; Dual luciferase flash - enabled by dispenser option; Nucleis acid/protein quantitation - photometric technology.

Make: Perkin Elmer

Form: Enspire Plate Reader UV/Vis/Fluorescent Service Form

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UVP BioDoc-it® No EtBr

Location: PSC 543, 659, NSC 338, NSC 460

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, Autorad film, colony plates and Fluorescent Gels. This self-contained gel imaging system features a scientific grade 2.0 megapixel CCD camera for capturing high resolution images with simple yet versatile capture and save options. Screen tilts to optimum viewing angle. A built-in computer touch screen supplies a complete system at one location for fluorescent and colorimetric imaging, while the attached comp.

NO ETHIDIUM BROMIDE is to be used in this instrument

Make: UVP

Form: UVP old EtBr OK Service Form

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AKTA Purifier

Location: PSC 542

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:ÄKTA™Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form: AKTA Purifier Accessories

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