Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:ÄKTA™ Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form/Manual:

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ÄKTA start is an easy-to-use chromatography system that automates the rapid, manual purification procedures using small volume chromatography columns, for example HiTrap columns.

Further Information: GE Healthcare

Make: GE Healthcare

Form/Manual:

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Ion OneTouch™ 2 System simplifies the workflows for the Ion Proton™ and Ion PGM™ systems by providing an automated solution for scalable and reproducible template preparation. The Ion OneTouch™ 2 System: • Integrates multiple manual template preparation workflows into a single system
• Enables parallel processing of multiple samples per day through modular design
• Supports scalable template preparation for all Ion semiconductor sequencing chips
• Supports up to 400 bases on the Ion PGM™ System and up to 200 bases on the Ion Proton™ System*
• Includes a full thermal cycling modality for amplification for quick optimization of thermal cycling parameters
• Employs a peristaltic pump fluidic system that eliminates the need for gas
• Fits easily on any benchtop with its small instrument footprint

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The IonS5 System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any lab or clinic. The Ion GeneStudio S5 Series is flexibly designed to enable a broad range of targeted NGS RNA/DNA sequence applications with industry-leading speed and scalability. Select from five different sequencing chips to sequence a throughput range from 2M to 130M reads per run. Simply choose the chip size and the instrument that matches your throughput and application needs.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Ion Chef™ System is the next generation of workflow simplification products for the Ion Proton™ and Ion PGM™ systems. The Ion Chef™ System provides automated, high-throughput template preparation and reproducible chip loading for users at any experience level. With typically less than 15 minutes of hands-on time, an intuitive touch-screen interface, and significantly higher productivity than the Ion OneTouch™ 2 System, the Ion Chef™ System makes the Ion Torrent™ sequencing workflow accessible for every lab.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Ping Jiang / Sonja Young

Administrator Contact: (404) 413-5370

Application: The Ion Proton™ System offers a complete workflow from sample through analysis, and variant calling or RNA analysis. It is used with the Ion Chef™ System* to enable automated, high-throughput template preparation and reproducible chip loading for users of any experience level. Built for speed, the Ion Proton™ System provides sequencing run times from 2–4 hours on the Ion PI™ Chip, taking you from DNA to variants in a single day. It is equipped with Ion Reporter™ Software, providing integrated tools for tertiary data analysis for DNA variation analysis across single, paired, or trio research samples.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Sonja Young

Administrator Contact: (404) 413-5370

Application: The nCounter SPRINT platform is capable of degitally analyzing multiple pathways in a single tube in an extraction-free workflow requiring only 10 minutes of hands-on time -significantly benefiting your RNA, DNA, and protein profiling applications.

Make: Nanostring Technologies

For Use and Further Information:

For Basic Use Capabilities: Online Information

Administrator: Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: The BD Accuri™ C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable. The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. As a result, flow cytometry has become a staple of modern laboratories around the world.

Make: BD Biosciences

Form: Accuri C-6 Blue/Red Request for Service Form

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: Simple Western by ProteinSimple effectivley reinvents the entire Western blot process without gels, transfer tanks, blots or film - finally giving researchers a complete, walk-away solution for protein detection and characterization. All hands-on steps from sample loading and detection to quantitative data analysis are completely automated, eliminating the manual factors that can negatively impact reproducibility, quantitation, time to result and data reliability. Simon analyzes 12 samples per run in 3-5 hours! Funding provided by Tech fee.

Make: Protein Simple

Form: Protein Simple Simon Western Service Form

Administrator: Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: This benchtop system delivers high-speed sorting and multicolor analysis. The BD FACSAria instrument is the first benchtop sorter that incorporates a fixed-alignment cuvette flow cell, which minimizes startup time and improves reproducibility. This new flow cell provides superior fluorescence sensitivity and freedom from instrument maintenance and alignment. Gel coupling to the objective lens improves collection efficiency and increases sensitivity and resolution needed ofr multicolor sorting applications. Additional "Sort setup" and "sort monitoring" features in the system software makes the BD FACSAria instrument easy to use. BD FACS™ Accudrop technology is integrated into the system for quick and accurate drop-delay determination. Stream monitoring of the breakoff point and clog detection are incorporated. The system has four air-cooled lasers at 488-nm, 633-nm, 405-nm and 355-nm wavelengths. Two- and four-way bulk sorting devices for a variety of tube sizes are also available. Water Jacketed tube holders are also available to keep cells warm or cold as needed.

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

Form: Aria Cell Sorter Blue/Red/Violet/UV Service Form

Administrator: Ping Jiang and "Sandy" Ying Sin Hsieh

Administrator Contact: (404) 413-5363

Application: The Nanophotometer is an All-in-One spectrophotomer with micro volume (0.3 ul) and optimized for quantification and purity assessment of nucleic acid and protein samples. Sample Compression Technology allows for accurate (+/- 5%) and reproducible (.005 Abs) readings of nucleic acids, proteins and peptides. This technology also is tolerant of a wide range of buffers that often interfere with an accurate reading. Funding provided by Tech fee.

Make: Implen

Form: Nanophotometer Operation Manual

Administrator: Ping Jiang

Administrator Contact: 404-413-5379

Application: The Qubit™ 3.0 Fluorometer is the next generation of the popular benchtop fluorometer that accurately measures DNA, RNA, and protein using the highly sensitive Qubit quantitation assays. The concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to to the target, which minimizes the effects of contaminants—including degraded DNA or RNA—on the result. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds Key features of the Qubit 3.0 Fluorometer include: Powerful, dual-core processor quickly and accurately quantifies DNA, RNA, and protein, in less than 5 seconds per sample Uses as little as 1 μL of sample Stores up to 1,000 sample results Large 5.7-inch state-of-the-art color touch screen for easy workflow navigation Ability to personalize your Qubit Fluorometer with the assays you run most, add new assays, or even create your own with MyQubit

Make:Thermofisher

Form/Manual: Qubit 3 Fluorometer

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Exceptional sensitivity for quantitation of biomolecules labeled with chemiluminescent probes Supports gel documentation of Coomassie™ blue and silver-stained samples. Accurate quantitation of closely spaced bands in complex protein samples for gels and films up to 18 x 12 cm. Low noise for longer exposure times and lower background, important for precise quantitation of very weak signals. Wide linear dynamic range for precise quantitation of faint and dark bands over four orders of magnitude in the same image, avoiding time-consuming multiple film exposures. Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision and uniform quantitation. Sensitive detection of less than 40 pg of protein on a Western blot. Sensitivity may be increased by "binning" of up to 8 x 8 pixels and smoothing. Chemiluminescent and colorimetric signals can be captured in the same image without changing the lens.

Make: GE Healthcare

Form: LAS4000 mini Chemiluminescence Service Form

Administrator: "Sandy" Ying Sin Hsieh or Liana Artinian

Administrator Contact: (404) 413-5363 or (404) 413-5345

Application: TILL Photonics Imaging system offers advanced equipment used for a variety of different applications studying dynamic processes in living cells such as:

•Measurement of rapidly changing intracellular ion concentrations (Ca2+, Na+ and other ions) e.g. in neurons.
•Study of signal transduction processes in response to e.g. growth factors or cytokines.
•Study of calcium sensitive processes.
•Simultaneous imaging of different target molecules within one sample using multiple staining with different dyes or probes.
•Metabolic transport.
•Cell-cell interactions.

Make: TILL Photonics

Form: TILL Photonics Imaging System Service Form

Administrator: Robert Simmons and Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: The STP-120 Spin Processor embeds the tissue in paraffin.

Make: Fisher

Form: STP-120 Spin Processor Service Form

Administrator: Robert Simmons and Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: The Histocenter 3 embeds tissue samples into paraffin blocks for microtome sectioning.

Make: Shandon

Form: Histocentre 3 Service Form

Administrator: Robert Simmons and Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: IMEB.inc

Form: Microtome Model 86C Sliding Service Form

Administrator: Robert Simmons and Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: Unknown

Form: Rotory Microtome Service Form

Administrator: Robert Simmons and Sandy Hsieh

Application: The HM550 Microtome esnures that sectioning of paraffin blocks into extremely thin slices proceeds at an optimal sectioning temperature

Make: Fisher

Form: HM 550 Microtome Service Form

Administrator: "Sandy" Ying Sin Hsieh and Hyuk Kyu Seoh

Administrator Contact: 404-413-5379

Application: You can access pre-stored calculations such as ratios which provide double emission readout in a FRET-based or cell viability assay. You can run endpoint assays with one or two wavelengths or scan the entire wavelength range. You can also follow enzyme kinetics in real time.

Other applications: Enzyme kinetics - Temperature controlled reagent reservoirs with plate temperature control for stability and accuracy. Can be integrated with PerkinElmer automated liquid handling platforms; Cellular - Well area scanning. Can be integrated with PerkinElmer automated liquid handling platforms; Viable cell - Optional bottom reading offering signicant improvements for viable cell assays; ELISA - Photometric technology and AlphaLISA allow conversion to homogenous ELISA technology; GFP and other fluorescent proteins - Quad monochromators, bottom reading and well scanning; Luminescent Reporter gene and cell growth - Ultra-sensitive luminescence with dynamic range for low transfection rates; Calcium - dispenser for suspension cells convenient in receptor activation studies; Dual luciferase flash - enabled by dispenser option; Nucleis acid/protein quantitation - photometric technology.

Make: Perkin Elmer

Form: Enspire Plate Reader UV/Vis/Fluorescent Service Form

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, Autorad film, colony plates and Fluorescent Gels. This gel imaging system features a scientific grade 2.0 megapixel CCD camera for capturing high resolution images with simple yet versatile capture and save options on the attached comp.

NO ETHIDIUM BROMIDE is to be used in this instrument

Make: UVP

Form: UVP old EtBr OK Service Form

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, Autorad film, colony plates and Fluorescent Gels. This self-contained gel imaging system features a scientific grade 2.0 megapixel CCD camera for capturing high resolution images with simple yet versatile capture and save options. Screen tilts to optimum viewing angle. A built-in computer touch screen supplies a complete system at one location for fluorescent and colorimetric imaging, while the attached comp.

NO ETHIDIUM BROMIDE is to be used in this instrument

Make: UVP

Form: UVP old EtBr OK Service Form

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5363

Application: The Nanophotometer is an All-in-One spectrophotomer with micro volume (0.3 ul) and optimized for quantification and purity assessment of nucleic acid and protein samples. Sample Compression Technology allows for accurate (+/- 5%) and reproducible (.005 Abs) readings of nucleic acids, proteins and peptides. This technology also is tolerant of a wide range of buffers that often interfere with an accurate reading. Funding provided by Tech fee.

Make: Implen

Form/Manual: Nanophotometer Operation Manual

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:ÄKTA™Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form: AKTA Purifier Accessories

Administrator: Sandy Hsieh

Administrator Contact: (404) 413-5363

Application: QuantStudio3™ Real-Time PCR Systems offer intuitive software, Fast (<40 min) and standard (<2 hr) runs, and are configured for PC-free or networked operation with intuitive software ineterface. These factory-calibrated systems make it easy to step up to high-performance qPCR using Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.

Make: Life Technologies

Form/Manual: QuantStudio3 Operation Manual