Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Agilent's GeneSpring GX software provides powerful, accessible statistical tools for fast visualization and analysis of microarrays - expression arrays, miRNA, exon arrays and genomics copy number data. Designed specifically for the needs of biologists, GeneSpring GX offers an interactive desktop computing environment that promotes investigation and enables understanding of microarray data within a biological context.

Make: Agilent Technologies

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: FlowJo is a software package that is capable of analyzing flow cytometry data. All files produced by assorted flow cytometers are written in the Flow Cytometry Standard format with an .fcs file extension. FlowJo will import and analyze cytometry data regardless of which FACS (Fluorescence Activated Cell Sorting) insrument/software is used to collect the data.

To use the Software please contact an ABCore Technician, who will explain how to download the software to your computer. The software access privileges are sometimes donglebased, and therefore will require you to sign-out for a dongle from PSC 521 or come to some other arrangements with Flow-Jo.

Make: FlowJo

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: DeCyder 2-D Differential Analysis Software is specifically designed for 2-D fluorescence difference gel electrophoresis (DIGE) analysis and is a key element in the Ettan DIGE system (which we have). It is fullyully optimized and integrated to the 2-D DIGE platform.

Make: GE Healthcare Life Sciences

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ImageMaster 2D software offers a flexible interface for the comprehensive visualization, exploration, and analysis of 2-D gel data from both 2-D DIGE (2-D fluorescence difference gel electrophoresis) and non-DIGE technologies. It is provided as altrenative to the use of Decyder 2D amalysis Software

Make:GE Healthcare Life Sciences

Form/Manual: ImageMaster 2D Proteomics Analysis Manual

Administrator: an ABCore Technician or Sonja Young

Administrator Contact: 404-413-5363

Application: The importance of sterilization is essential to good laboratory practice, particularly in biotechnology, pharmaceutical and clinical laboratories. Of all sterilization methods, high pressure steam (autoclave) is the most widely used because of its efficacy, speed and reliability. The department has a number of autoclaves, some run by steam and others use steam, but the steam is generated by water loaded into the autoclave and electricity.

Autoclaves are used in a wide variety of applications including:

• culture media preparation
• labware sterilization
• waste sterilization

Make: Steris/AMSCO

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Thermo Electron offers the FRENCH Press and two models of FRENCH Pressure Cells for the disintegration of chloroplast materials, blood cells, unicellular organisms, homogenates of animal tissue and other biological particles. The pressure cell disrupts the cellular walls of a sample while leaving the cell nucleus undisturbed. This technique results in more uniform and complete disruption. The FRENCH Pressure Cell delivers higher pressures than bombs or decompression systems, without pre-treating the sample. Cells are lysed by forcing the cell or tissue suspension through a narrow space, thereby shearing the cell membraneøs.A French press consists of a piston that is used to apply high pressure to a sample volume of 40 to 250 ml, forcing it through a tiny hole in the press. It can reach pressures of 20,000-40,000 pounds per square inch (psi).

Make: Thermo Electron

Form/Manual: French Press Operation Manual

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: The Sonifier® ultrasonic cell disruptors are versatile laboratory tools designed to apply high-frequency ultrasonic energy to biological and chemical processing to provide:

• Cell disruption
• Nano particles
• Emulsifications
• Homogenization
• Cell lysis
• Processing DNA & proteins
• Fine mixing
• Degassing

Make: Fisher / Emerson Industrial Automation

Form/Manual: Sonicator Tip Operations Manual

Administrator: Hyuk_Kyu Seoh

Administrator Contact: (404) 413-5379

Application: The Sonifier® ultrasonic cell disruptors are versatile laboratory tools designed to apply high-frequency ultrasonic energy to biological and chemical processing to provide:

• Cell disruption
• The bath adapter allows for the simultaneous sonication. didruption of cells surrounded by ice water, to • unify cell disruption

Make: Fisher / Emerson Industrial Automation

Form/Manual: Sonicator (Bath) Operations Manual

Administrator: George Pierce

Administrator Contact: 404-413-5315

Application: This system allows for continuous temperature control constantly maintaining cell suspensions at a low temperature. It offers a controlled cell breakage rate, and direct steam sterilization and flushing with antiseptics (DynoMill brochure).

Make: DynoMill

Form/Manual: KDL Agitator Bead Mill Service Form

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-20 has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-20 Floor Operation Manual

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-26 XP has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-26 XP Floor Operation Manual

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-25 has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-25 Floor Operation Manual

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The particle’s settling velocity in centrifugation is a function of the particle’s size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity. The Avanti J-25I has a fast accel/decel rate and can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Beckman

Form/Manual: Avanti J-25-I XP Floor Operation Manual

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The Sorvall RC5B can process large volumes of solutions. The rate of separation depends on the rotor radius and type.

Make: Sorvall

Form/Manual: Operation Manual

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Centrifuge 5804 is a high speed centrifuge for medium capacity needs. It allows for molecular applications in tubes up to 250 mL and offers additional swing-bucket and fixed-angle rotors as well as deepwell plate capacity for increased versatility.

Make: Eppendorf

Form/Manual: 5804-Refrigerated Tabletop Operation Manual

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Centrifuge 5804 R is a high speed, refigerated centrifuge for medium capacity needs. It allows for molecular applications in tubes up to 250 mL and offers additional swing-bucket and fixed-angle rotors as well as deepwell plate capacity for increased versatility.

Make: Eppendorf

Form/Manual: 5804-Refrigerated Tabletop Operation Manual

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Centrifuge 5810 and 5810R are -high speed centrifuges for medium capacity needs. They allow for molecular applications in tubes up to 250 mL and offer additional swing-bucket and fixed-angle rotors as well as deepwell plate capacity for increased versatility.

Make: Eppendorf

Form/Manual: 5810 R Tabletop Operation Manual

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The Centrifuge 5430 R combines the proven features of the Eppendorf microcentrifuges and the multipurposecentrifuges. They offer multiple application opportunities due to a high selection of different rotor types. Despite their compact size of approx. 35 cm width, special rotors for micro plates, 15 mL and 50 mL conical tubes can be easily used. Their incomparable versatility makes the Centrifuge 5430 and Centrifuge 5430 R the flagships under the Eppendorf microcentrifuges.

Make: Eppendorf

Form/Manual: 5430 R Tabletop Operation Manual

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ÄKTA™Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form/Manual:

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ÄKTA™Pure chromotography systems are designed for the rapid development and optimization of biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Further Information: GE Healthcare

Make: GE Healthcare

Form/Manual:

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:ÄKTA™ Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form/Manual:

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: ÄKTA start is an easy-to-use chromatography system that automates the rapid, manual purification procedures using small volume chromatography columns, for example HiTrap columns.

Further Information: GE Healthcare

Make: GE Healthcare

Form/Manual:

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application:ÄKTA™Purifier and Explorer chromotography systems are designed for fast development and optimization of any biomolecular purification, using one working platform for all techniques and samples.

Applications: The method wizard and optimized system configurations, allows rapid media screening, method scouting, method optimization, and scaleup experiments. Standard system configurations are available to suit most purification needs, but are easily modified if required.
• Protein, peptide, nucleic acid purification in micro and gram scales
• Programmable sequential application of different columns up to 8
• Automated sample load up to 8 different samples or sequential load of samples
• Automated fraction collection in bulk or small fractions
• BufferPrep calculates and prepares the correct pH buffers
• Samples can be applied at flow rates up to 50 ml/min. and fractions can be collected without spillage at flow rates up to 100 ml/min.

Make: GE Healthcare

Form/Manual:

Administrator: Hyuk Kyu Seoh

Administrator Contact: (404) 413-5379

Application: The MDQ is a capillary-based analytical system separates sample components within a fused-silica capillary, using one of several modes of electrophoresis.

Applications: Examples of the different modes of electrophoresis include:
• Capillary Zone Electrophoresis
• Micellar Electrokinetic Capillary Chromatography
• Capillary Gel Electrophoresis
• Capillary Isoelectric Focusing
• Capillary Electrochromatography.
The system includes a P/ACE MDQ configured with both a photo diode array and selectable-wavelength UV/Vis (200, 214, 254 and 280 nm filters included) detector, UV source optics, temperature-controlled sample storage module and 32 Karat™ Software. Typical applications are:
• Genetic analysis
• Chiral analysis
• Fast RNA purity analysis
• Basic drugs analysis
• Ion analysis
• Protein and Peptide analysis

Make: Beckman

Form/Manual: MDQ Service Form

Administrator: Hyuk Kyu Seoh

Administrator Contact: 404-413-5379

Application: The Beckman HPLC has solvent delivery modules that are built with a rapid fill pump design that produce smooth flow from microbore to semi-preparative flow rates and thee high-pressure mixing design eliminates the need for constant helium sparging or degassing. The pumps are combined with high-sensitivity UV/VIS and diode array detectors and autosamplers. Flow range: 0.01 - 10.0 ml/min.; wavelength range: 190 - 700 nm; Maximum back pressure: 17 ul capacity flow cell; Programmable functions: wavelength, range, rise time, auto zero, event mark, relays, lamp off, peak detection, peak threshold, bandwidth and stop data.

Preparative HPLC refers to the process of isolation and purification of compounds. Important is the degree of solute purity and the throughput, which is the amount of compound produced per unit time. This differs from analytical HPLC, where the focus is to obtain information about the sample compound. Chemical Separations can be accomplished using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase. Each compound should have a characteristic peak under certain chromatographic conditions. Depending on what needs to be separated and how closely related the samples are, the chromatographer may choose the conditions, such as the proper mobile phase, to allow adequate separation in order to collect or extract the desired compound as it elutes from the stationary phase. With these characteristic peaks, identification of compounds can be done. HPLC also allows for quantification of compounds by determining the unknown concentration of a compound in a known solution.

Make: Beckman

Form/Manual: HPLC Service Form

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The Concentrator plus from Eppendorf is available in two configurations:

Complete system with an integrated service-free diaphragm pump
Basic concentrator for connecting to an existing vacuum.
The Concentrator’s heating technology optimizes the evaporation process. No matter which configuration you choose, you’re assured of quick and efficient—yet gentle—vacuum concentration of your samples (e.g. DNA/RNA, proteins).

The complete system includes a vapour condenser that purifies the exhaust air by up to 85%. This serves as protection against unpleasant vapours.

Make: Eppendorf

Form/Manual: Speed Vac Concentrator Operation Manual

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: The Vacufuge Concentrator is a complete system with solvent-resistant PTFE vacuum pump and vapor trap. Narrow, 13-inch width saves valuable bench space. Motorless drive and sealed chamber provide trouble-free operation Evaporation Modes:

• Choice of three (aqueous, alcohol, off) to correspond with sample solvent and reduce processing time up to 20%.
• Maximize evaporation efficiency and prolong pump lifespan by periodically aerating the vacuum line, preventing condensation build-up.

Automatic vacuum release prevents sample loss from bumping and foaming. Brake feature brings rotor to a faster stop—allows quick access to samples. Choice of four heating levels (30°C, 45°C, 60°C, and ambient) allows safe and efficient concentration of a variety of samples. Besides the microfuge tube rotor, there is a micro plate rotor available upon request (see Sandy).

Make: Eppendorf

Form/Manual: Vacufuge Operation Manual

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5379

Application: Washing Dishes

Make: Steris/AMSCO

Form/Manual: Dishwasher Operation Manuals

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application:The 24-capillary 3500xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Make: Life Technologies

For Use and Further Information: DNA Sequencing Services

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The Ion OneTouch™ 2 System simplifies the workflows for the Ion Proton™ and Ion PGM™ systems by providing an automated solution for scalable and reproducible template preparation. The Ion OneTouch™ 2 System: • Integrates multiple manual template preparation workflows into a single system
• Enables parallel processing of multiple samples per day through modular design
• Supports scalable template preparation for all Ion semiconductor sequencing chips
• Supports up to 400 bases on the Ion PGM™ System and up to 200 bases on the Ion Proton™ System*
• Includes a full thermal cycling modality for amplification for quick optimization of thermal cycling parameters
• Employs a peristaltic pump fluidic system that eliminates the need for gas
• Fits easily on any benchtop with its small instrument footprint

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The IonS5 System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any lab or clinic. The Ion GeneStudio S5 Series is flexibly designed to enable a broad range of targeted NGS RNA/DNA sequence applications with industry-leading speed and scalability. Select from five different sequencing chips to sequence a throughput range from 2M to 130M reads per run. Simply choose the chip size and the instrument that matches your throughput and application needs.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The Ion Chef™ System is the next generation of workflow simplification products for the Ion Proton™ and Ion PGM™ systems. The Ion Chef™ System provides automated, high-throughput template preparation and reproducible chip loading for users at any experience level. With typically less than 15 minutes of hands-on time, an intuitive touch-screen interface, and significantly higher productivity than the Ion OneTouch™ 2 System, the Ion Chef™ System makes the Ion Torrent™ sequencing workflow accessible for every lab.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The Ion Proton™ System offers a complete workflow from sample through analysis, and variant calling or RNA analysis. It is used with the Ion Chef™ System* to enable automated, high-throughput template preparation and reproducible chip loading for users of any experience level. Built for speed, the Ion Proton™ System provides sequencing run times from 2–4 hours on the Ion PI™ Chip, taking you from DNA to variants in a single day. It is equipped with Ion Reporter™ Software, providing integrated tools for tertiary data analysis for DNA variation analysis across single, paired, or trio research samples.

Make: Life Technologies (Thermofisher)

For Use and Further Information: DNA Sequencing Services

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The nCounter SPRINT platform is capable of degitally analyzing multiple pathways in a single tube in an extraction-free workflow requiring only 10 minutes of hands-on time -significantly benefiting your RNA, DNA, and protein profiling applications.

Make: Nanostring Technologies

For Use and Further Information:

For Basic Use Capabilities: Online Information

Administrator: an ABCore Technician

Administrator Contact: 404-413-5363

Application: This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system is optimized for the most popular range of separations with a fixed angle of 120°. This unit can be used for separations of fragments up to 6 Mb in size by adjusting the running conditions for low voltage and extended run times. Besides mapping, this system can be used for:

• Strain typing-molecular epidemiology
• Apoptosis assays
• DNA damage and repair studies
• Large protein separations

Pulsed Field Gel Electrophoresis Is Still the Benchmark for Mapping Applications such as: • Disease locus mapping

• YAC, BAC, PAC, and cosmid mapping
• Chromosome rearrangements
• RFLP and DNA fingerprinting

Make: BioRad

Form/Manual: CHEF DR II Pulse Wave

Administrator: Hyuk-Kyu Seoh

Administrator Contact: (404) 413-5379

Application: Simple Western by ProteinSimple effectivley reinvents the entire Western blot process without gels, transfer tanks, blots or film - finally giving researchers a complete, walk-away solution for protein detection and characterization. All hands-on steps from sample loading and detection to quantitative data analysis are completely automated, eliminating the manual factors that can negatively impact reproducibility, quantitation, time to result and data reliability. Simon analyzes 12 samples per run in 3-5 hours! Funding provided by Tech fee.

Make: Protein Simple

Form/Manual: Protein Simple Simon Western

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application:T he Gene Pulser Xcell™ system is a modular electroporation system for transfecting every cell type. The system includes a main unit, a ShockPod™ cuvette chamber, and your choice of accessory modules: the capacitance extender (CE module) and the pulse controller (PC module).

Features and Benefits:
• Universal electroporation — transfects all cell types, from primary and stem cells to bacteria and yeast
• Preset protocols — include the most common mammalian and bacterial cell types
• Flexibility — choice of programs for preset protocols, optimization protocols, manual operation, and/or user protocols
• Protocol library — collection of electroporation protocols for every cell type including primary, • immortal, and bacterial cells
• Data management — enables storage and recall of parameters used in the previous 100 experiments, useful for troubleshooting
• Reproducibility — uses PulseTrac™ circuitry and arc-protection features to ensure reproducibility and sample protection

Make: BioRad

Form/Manual: Gene Pulser Xcell

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: The MicroPulser electroporator is a simple yet versatile instrument that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms. Transformation efficiencies much higher than those obtained with chemical methods can be achieved.
Key Features:
• One-button pulse delivery, attached cuvette chamber, and rapid charge time for fast sample handling
• Preset, optimized programs for commonly studied bacteria and fungi allow rapid program selection
• Arc quenching (ARQ) system significantly reduces arcing, protecting against loss of valuable samples
• Broad range of parameters for manual optimization. Manual programming allows voltage to be selected • in a 200–3,000 V range with 10 V precision and allows pulse width to be selected in a 1.0–4.0 ms range with 0.1 ms precision
• High-voltage 3,000 V capability yields improved efficiency in larger-volume cuvettes
• Compact, space-saving design
• Audible and visible pulse indicators
• Time constant and actual volts displayed to monitor reproducibility

Make: BioRad

Form/Manual: Micropulser Operation Manual

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The X-OMAT 2000A processes exposed radiographic film. The exposed film sheet is placed into an automatic film processor. The film is processed via rollers through tanks containing developer and fixer solutions, and finally through a wash system. The developed film emerges from the processor ready for reading by the researcher.

Make: Kodak

Form/Manual: X-Omat 2000A Operation Manual

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: The BD Accuri™ C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable. The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. As a result, flow cytometry has become a staple of modern laboratories around the world.

Make: BD Biosciences

Form/Manual: Accuri C-6 Blue/Red

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: This benchtop system delivers high-speed sorting and multicolor analysis. The BD FACSAria instrument is the first benchtop sorter that incorporates a fixed-alignment cuvette flow cell, which minimizes startup time and improves reproducibility. This new flow cell provides superior fluorescence sensitivity and freedom from instrument maintenance and alignment. Gel coupling to the objective lens improves collection efficiency and increases sensitivity and resolution needed ofr multicolor sorting applications. Additional "Sort setup" and "sort monitoring" features in the system software makes the BD FACSAria instrument easy to use. BD FACS™ Accudrop technology is integrated into the system for quick and accurate drop-delay determination. Stream monitoring of the breakoff point and clog detection are incorporated. The system has four air-cooled lasers at 488-nm, 633-nm, 405-nm and 355-nm wavelengths. Two- and four-way bulk sorting devices for a variety of tube sizes are also available. Water Jacketed tube holders are also available to keep cells warm or cold as needed.

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

For Use and Further Information: Flow Cytometry and Cell Sorting Services

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results.

The BD FACSCanto II provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors.*

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

For Use and Further Information: Flow Cytometry and Cell Sorting Services

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: The Auto 2000 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Make: Nexcelom

Form/Manual: Cellometer Cell Counter

Administrator: an ABCore Technician

Administrator Contact: (404) 413-5363

Application: The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

The Fortessa is configurable and upgradeable with up to 4 lasers to detect up to 18 colors simultaneously, BD LSR analyzers feature innovative designs for both the excitation optics and collection optics that reduce excitation losses and improve light collection efficiency. Our instrument has blue (488 nm), red (633 nm), yellow-green (561 nm) and violet (405nm) lasers. The addition of the yellow-green laser allows use of the new fruit fluorescent proteins such as mCherry. It also limits spectral overlap between FITC and PE – 2 of the most common fluorescent markers used in flow cytometry.

Applications are far reaching and can be found on the BD online tutorial.

Make: BD Biosciences

For Use and Further Information: Flow Cytometry and Cell Sorting Services

Administrator: Sonja Young and an ABCore Technician

Administrator Contact: (404) 413-5363

Application: Maintain viable stocks and strains at -80°C

Make: Thermo

Form/Manual: Freezer Manual

Administrator: Sonja Young and an ABCore Technician

Administrator Contact: (404) 413-5363

Application: Explosion Proof -20ºC

Make: Thermo

Form/Manual: Freezer

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: 404-413-5363 and 404-413-5379

Application: Sublimation occurs when a frozen sample goes directly to the gaseous phase without passing through the liquid phase. As the ice sublimates, it leaves voids in the dry residual material making it easy to rehydrate. Freeze drying samples stabilizes them for storage, preservation, or shipping . Many times, drying causes damage and some loss of cellular or protein activity. Lyophilization is a method of sample preservation that significantly reduces damage to biological samples. Substances that are not damaged by freezing can usually be lyophilized so that refrigerated storage is unnecessary. Important exceptions are mammalian cells, nearly all of which are destroyed by lyophilization. However, many microorganisms and proteins survive lyophilization well, and it is a preferred method of drying vaccines, pharmaceuticals, blood fractions, and other laboratory samples. Some specialist food products are also lyophilized.

Make: Virtis

Form/Manual: BT3.3 EL Lyophilizer Tabletop Service Form

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: Sublimation occurs when a frozen sample goes directly to the gaseous phase without passing through the liquid phase. As the ice sublimates, it leaves voids in the dry residual material making it easy to rehydrate. Freeze drying samples stabilizes them for storage, preservation, or shipping . Many times, drying causes damage and some loss of cellular or protein activity. Lyophilization is a method of sample preservation that significantly reduces damage to biological samples. Substances that are not damaged by freezing can usually be lyophilized so that refrigerated storage is unnecessary. Important exceptions are mammalian cells, nearly all of which are destroyed by lyophilization. However, many microorganisms and proteins survive lyophilization well, and it is a preferred method of drying vaccines, pharmaceuticals, blood fractions, and other laboratory samples. Some specialist food products are also lyophilized.

Make: LabConco

Form/Manual: Freezone Large Service Form

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: Sublimation occurs when a frozen sample goes directly to the gaseous phase without passing through the liquid phase. As the ice sublimates, it leaves voids in the dry residual material making it easy to rehydrate. Freeze drying samples stabilizes them for storage, preservation, or shipping . Many times, drying causes damage and some loss of cellular or protein activity. Lyophilization is a method of sample preservation that significantly reduces damage to biological samples. Substances that are not damaged by freezing can usually be lyophilized so that refrigerated storage is unnecessary. Important exceptions are mammalian cells, nearly all of which are destroyed by lyophilization. However, many microorganisms and proteins survive lyophilization well, and it is a preferred method of drying vaccines, pharmaceuticals, blood fractions, and other laboratory samples. Some specialist food products are also lyophilized.

Make: LabConco

Form/Manual: Freezone Large Service Form

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: LabConco

Form/Manual: Freeze Dry System 4 Small Service Form

Administrator: Sonja Young and Hyuk Kyu Seoh

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: LabConco

Form/Manual: Lyophilizer Small Service Form

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: LabConco

Form/Manual: Lyophilizer Small with Dry Ice Tap Service Form

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Model 583 Gel Dryer allows the drying of acrylamide and other gel types.
The Model 583 gel dryer has a 35 x 45 cm drying surface, which is large enough to fit up to 16 mini gels, 9 Criterion gels, 2 large format gels, or 1 sequencing gel.

Make: BioRad

Form/Manual:

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Leveraging technologies adapted from the semiconductor industry, Affymetrix, the principle manufacture of GeneChip arrays uses photolithography and solid-phase chemistry to produce arrays containing hundreds of thousands of oligonucleotide probes packed at extremely high densities. The probes are designed to maximize sensitivity, specificity, and reproducibility, allowing consistent discrimination between specific and background signals, and between closely related target sequences.
Attesting to their powerful capabilities, GeneChip arrays are applied in a wide variety of DNA and mRNA analyses. Recent analytical accomplishments include the elucidation of interactions between signaling pathways involved in development, the discovery of a new class of leukemia, and the development of new assays to track drug metabolism.

Make: Affymetrix

For Use and Further Information: Genomics Microarray Services

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The GeneChip® Fluidics Station 450 is used for the wash and stain operation of Affymetrix GeneChip® arrays. The instrument includes design advancements to provide improved ease of use and true walk-away freedom dramatically improving efficiency in your genetic analysis.

Make: Affymetrix

For Use and Further Information: Genomics Microarray Services

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: GeneChip® Hybridization Oven provides uniform hybridization environmment for consistent RNA : chip bound DNA interactions, allowing for appropriate discrimination between specific and background signals, and between closely related target sequences.

Make: Affymetrix

For Use and Further Information: Genomics Microarray Services

Administrator: Chung Dar Lu and P.C. Tai

Administrator Contact: (404) 413-5363

Application: DNA Microarrays and Proteomic Technologies allow scientists to detect genes or proteins that are coregulated and whose patterns of change correlate with something important such as a disease state. However there is no assurance that these changes are really significant to the cell. Phenotype MicroArrays are a complementary technology providing the needed information at the cellular level ... and much more. After a genetic change or exposure to a drug lead, the researcher can directly evaluate the cellular response to that change. Phenotype MicroArrays can monitor, either directly or indirectly, most known aspects of cell function. The range of phenotypes includes:

• Cell surface structure and transport functions
• Catabolism of carbon, nitrogen, phosphorus, and sulfur
• Biosynthesis of small molecules
• Synthesis and function of macromolecules and cellular machinery
• Cellular respiratory functions
• Stress and repair functions
• Other cellular properties

Make: Biolog

Form/Manual: Biolog Phenotype Microarray

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: The popular GenePix® 4000B Microarray Scanner has earned its outstanding reputation due to its incredible, intuitive, and reliable operation. Our commitment to engineering excellence guarantees that the GenePix 4000B Microarray Scanner remains the benchmark in microarray scanning technology. In 2010 we updated the electronics to provide a state-of-the-art microarray scanner with the finest quality components while maintaining the features and performance that you have become accustomed to.

Make: Molecular Devices

Form/Manual: GenePix MicroArray Scanner

Administrator: Robert Simmons and Sonja Young

Administrator Contact: (404) 413-5363

Application: The Histocenter 3 embeds tissue samples into paraffin blocks for microtome sectioning.

Make: Shandon

Form/Manual: Histocentre 3

Administrator: Robert Simmons and Sonja Young

Application: The HM550 Microtome esnures that sectioning of paraffin blocks into extremely thin slices proceeds at an optimal sectioning temperature

Make: Fisher

Form/Manual: HM 550 Microtome

Administrator: Robert Simmons and an ABCore Technician

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: IMEB.inc

Form/Manual: Microtome Model 86C Sliding

Administrator: Robert Simmons and an ABCore Technician

Administrator Contact: (404) 413-5363

Application: This microtome sections paraffin blocks into extremely thin slices using a sliding motion

Make: Unknown

Form/Manual: Rotory Microtome Service Form

Administrator: Robert Simmons and an ABCore Technician

Administrator Contact: (404) 413-5363

Application: The STP-120 Spin Processor embeds the tissue in paraffin.

Make: Fisher

Form/Manual: STP-120 Spin Processor Service Form

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The Bioanalyzer 2100 provides for a rapid visualisation of sample quality and quantity. High sensitivity with minimal sample quantities. Significant time savings - up to12 samples in 30 minutes. Reduced use and waste of hazardous chemicals.

Make: Agilent

Form/Manual: Bioanalyzer 2100

Administrator: Jesse Gardner

Administrator Contact: (404) 413-5370

Application: The Qubit™ 3.0 Fluorometer is the next generation of the popular benchtop fluorometer that accurately measures DNA, RNA, and protein using the highly sensitive Qubit quantitation assays. The concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to to the target, which minimizes the effects of contaminants—including degraded DNA or RNA—on the result. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds Key features of the Qubit 3.0 Fluorometer include: Powerful, dual-core processor quickly and accurately quantifies DNA, RNA, and protein, in less than 5 seconds per sample Uses as little as 1 μL of sample Stores up to 1,000 sample results Large 5.7-inch state-of-the-art color touch screen for easy workflow navigation Ability to personalize your Qubit Fluorometer with the assays you run most, add new assays, or even create your own with MyQubit

Make:Thermofisher

Form/Manual: Qubit 3 Fluorometer

Administrator: Hyuk-Kyu Seoh

Administrator Contact: 404-413-5379

Application: Exceptional sensitivity for quantitation of biomolecules labeled with chemiluminescent probes Supports gel documentation of Coomassie™ blue and silver-stained samples. Accurate quantitation of closely spaced bands in complex protein samples for gels and films up to 18 x 12 cm. Low noise for longer exposure times and lower background, important for precise quantitation of very weak signals. Wide linear dynamic range for precise quantitation of faint and dark bands over four orders of magnitude in the same image, avoiding time-consuming multiple film exposures. Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision and uniform quantitation. Sensitive detection of less than 40 pg of protein on a Western blot. Sensitivity may be increased by "binning" of up to 8 x 8 pixels and smoothing. Chemiluminescent and colorimetric signals can be captured in the same image without changing the lens.

Make: GE Healthcare

Form/Manual: LAS4000 mini Chemiluminescence

Administrator: an ABCore Technician

Administrator Contact: 404-413-5379

Application: Amersham Imager 600 is a sensitive and robust chemiluminescence imager for high-resolution digital imaging of protein and DNA samples in gels and membranes. Intuitive operation: You can operate the instrument from a tablet computer with easy-to-use image analysis software. No prior imager experience or training is required to obtain high-quality results. Excellent performance: Advanced optics provides high-resolution images, high sensitivity, and broad dynamic range. Robustness: An optimal instrument for multi-user labs.

Make: GE Healthcare

Form: LAS4000 mini Chemiluminescence Service Form

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Some applications on the Omega 10g are SYBR Green stained gels and GFP expressed experiments and Qdot multi-plexed western blots. The instrument has megapixel resolution, touch pad control panel, adjustable height sample tray, variable intensity UV Transilluminator and multi-wavelength LED Epi-Illumination.

Make: Ultra Lum

Form/Manual: Omega 10g

Administrator: an ABCore Technician

Administrator Contact: 404-413-5379

Application: Luminex 100 System can deliver a wealth of information accurately, inexpensively and in real-time enhancing your operational efficiency. This powerful, compact lab analysis system includes the Luminex 100, the XY Platform, and the SD. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays.

Make: Luminex

Form/Manual/website: Luminex 100 System

Administrator: an ABCore Technician

Administrator Contact: 404-413-5379

Application: The Luminex MagPix System has been recently purchased (Summer'19). It belongs to a family of Flow cytometric systems capable of multiplex analysis -potentially measuring up to 50 analytes in a single microplate well, using very small samples.

Administrator: an ABCore Technician and Sonja Young

Administrator Contact: (404) 413-5363

Application: The Mastercycler® family offers ultimate reliability combined with easy to use software. The universal block can work with all common consumables and increases the range of application flexibilities. The Mastercycler gradient has SteadySlope® technology ensuring optimal, constant heating and cooling rates, which means that results of gradient experiments can be transferred to routine applications.

Features:
• SteadySlope® and Triple Circuit Technology
•  Gradient block1: up to 20°C gradient span is possible at any step of the PCR program
•  Universal block2: Possible to use 0.2 mL tubes, 0.5 mL tubes or plates
•  Simple programming for gradient function, adjustable ramp rates, time and temperature increments, incubation mode, link function, pause function
•  Software-supported in situ Adapter
•  RS-232 and printer interface
•  Auto-restart option in the event of a power failure
personal card system

Make: Eppendorf

Form/Manual: Mastercycler Gradient Service Form

Administrator: an ABCore Technician and Sonja Young

Administrator Contact: (404) 413-5363

Application: The Mastercycler® family offers ultimate reliability combined with easy to use software. The universal block can work with all common consumables and increases the range of application flexibilities. The Mastercycler gradient has SteadySlope® technology ensuring optimal, constant heating and cooling rates, which means that results of gradient experiments can be transferred to routine applications.

• Advantages of Mastercycler pro:
• Ultimate reduction of evaporation
• Extremely fast heating and cooling rates
•  Gradient blocks with SteadySlope technology
•  Intuitive graphic programming
•  Display to indicate cycler number in a network
•  Optional self-test of peltier elements

Make: Eppendorf

Form/Manual: Mastercycler Gradient Pro Service Form

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Some of the separation Methods used in preparative ultracentrifugation:

Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima XL-100K Ultra Floor/a>

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima MAX Ultra Tabletop

Administrator: Sonja Young

Administrator Contact: (404) 413-5363

Application: Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima MAX-XP Ultra Tabletop

Administrator: Sonja Young

Administrator Contact:(404) 413-5363

Application: Some of the separation Methods used in preparative ultracentrifugation:

Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima TL Tabletop

Administrator: Sonja Young

Administrator Contact: 404-413-5363

Application: Differential centrifugation: pelleting. Start with a uniform mixture of sample – molecules or organelles in buffer. Centrifuge to obtain pellet of heavier molecules, and supernatant.

Density gradient centrifugation: Rate zonal (also termed sedimentation velocity, zone centrifugation). Use a continuous density gradient of solvent such as sucrose. Density increases toward the bottom of tube. Sample is layered on top. Centrifuge until molecules form discrete bands depending upon their sedimentation values. Generally stop the run before any of the molecules reaches the bottom. 100% separation is possible with a small sample. Separates molecules based on size (MW). Used to separate various types of macromolecules – mixture of proteins; different types of RNA; separate DNA from RNA, or proteins; ribosomal subunits; polyribosomes.

Density gradient centrifugation: Isopycnic centrifugation (also termed sedimentation equilibrium centrifugation). Mix gradient material (such as cesium chloride- CsCl) with sample molecules. During the centrifugation, the CsCl generates a gradient (“self-generating gradient”), and the molecules move to the position in the gradient where their density is the same as the gradient material. Requires long centrifugation time to form a gradient (36-48 hrs). Separates molecules based on their density – may have the same size. Often used to separate various types of DNA – circular vs linear, double stranded vs single stranded, DNA from RNA (RNA pellets), highly repetitive DNA (satellite DNA) from other DNA in the cell, can be used for separation of lipoproteins and cell organelles.

Make: Beckman

Form/Manual: Optima TLX Tabletop

Administrator: an ABCore Technician

Administrator Contact: 404-413-5363

Application: The Stratagene UV crosslinker is designed to crosslink DNA or RNA to Nylon, nitrocellulose, or nylon-reinforced mitrocellulose membranes after Northern or Southern blots, dot blotting and colony or plaque lifts. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80 °C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. Other applications include nicking ethidium bromide stained DNA in agarose gels and gene mapping ofr creating cleavage-inhibiting thymine dimers.

Make: Fisher

Form/Manual: UV Crosslinker

Administrator: an ABCore Technician

Administrator Contact: 404-413-5363

Application: The Milli Q has a 3 step purification process that produces water with extremely low ionic, organic, pyrogen and nuclease contamination; suitable for PCR, 2-D electrophoresis, DNA sequencing, cell culture and RNA applications. 
• Designed to specifically target contaminants detrimental to particular applications such as HPLC, molecular biology and ICP-MS.
• The systems incorporate a high precision resistivity monitor that records conductivity or resistivity (compensated or not at 25 °C), and also feature a built-in TOC monitor with a 1-999 ppb detection range for accurate analysis of organics. 
• Milli-Q resistivity and TOC meters are designed to take into account the suitability test requirements of USP 28-NF21.
• Includes a Pyrogard 5000 ultrafiltration cartridge for the production of pyrogen- and nuclease-free ultrapure water (<0.001 EU/mL), particularly important for life science or molecular biology applications. 
• The ultraviolet photo-oxidation process (UV at 254 and 185 nm) can effectively carry out the destruction of organic molecules, to reduce TOC levels to < 5 ppb, improving consistency and sensitivity in applications such as HPLC, IC, solid-phase extraction and UV spectroscopy. produces water with extremely low ionic, organic, pyrogen and nuclease contamination; suitable for PCR, 2-D electrophoresis, DNA sequencing, cell culture and RNA applications.

Make: Millipore

Form/Manual: Milli-Q Service Form

Administrator: an ABCore Technician

Administrator Contact: 404-413-5363

Application: The Milli Q IQ7000 is an ntelligent, intuitive water system that is designed to make research as pleasant and comfortable as possible — and to maximize lab productivity. The breakthrough ergonomic design and powerful purification media ensure consistent production of superior-quality ultrapure water. The Milli-Q® IQ 7000's ultraviolet photo-oxidation process (UV at 254 and 185 nm) can effectively carry out the destruction of organic molecules, to reduce TOC levels to < 5 ppb, improving consistency and sensitivity in applications such as HPLC, IC, solid-phase extraction and UV spectroscopy. produces water with extremely low ionic, organic, pyrogen and nuclease contamination; suitable for PCR, 2-D electrophoresis, DNA sequencing, cell culture and RNA applications.

Make: Millipore

Form/Manual: Milli-Q Service Form